| 赤霉素的分光光度法测定 |
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| 引用本文： | 肖志壮,邢红宏.赤霉素的分光光度法测定[J].烟台大学学报(自然科学与工程版),1997,10(4):265-268. |
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| 作者姓名： | 肖志壮 邢红宏 |
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| 作者单位： | 烟台大学生物化学系 |
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| 摘 要： | 赤霉素在体积分数为７０％乙醇溶液中，９８％（质量分数）硫酸作用生成的产物在４１２ｎｍ波长处具有最大的吸收峰；在０－２００ｍｇ／Ｌ浓度范围内，赤霉素浓度与其吸光度值呈线性关系，其相关系数大于０．９９０；与荧光分光光度法和紫外光分光光度法对照，本方法的可信度在９９％以上。
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| 关 键 词： | 赤霉素 分光光度法 测定 植物激素 |
A Spectrophotometric Method for the Determination of Gibberellin |
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| Abstract: | The solution of gibberellin in 70% ethanol reacting chemically with 98% H 2SO 4 has maximal absorbency at the wavelength of 412nm. The concentration of gibberellin is linear with its A 412 ranging from zero to 200 mg/L, and the relation coefficient is more than 0.990. Compared with that of ultraviolet spectrophotometry, the credity of this method is above 99%. |
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| Keywords: | gibberellin spectrophotometry |
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