95%除草啶原药90天喂养对大鼠的毒性实验研究

观察除草啶经口饲喂90 d对大鼠血液、尿液、脏器重,生化指标及病理组织学的变化。依据GB15670-1995《农药登记毒理学试验方法》对其进行了为期90 d的毒性实验。结果,试验期间各组动物一般情况良好,无死亡、厌食和其它异常行为;中、高剂量组雄、雌性大鼠周平均体重从第2周开始至实验结束均明显低于对照组(p<0.05,p<0.01);高剂量组雄、雌性大鼠的食物消耗量明显低于对照组(p<0.05)。血液学检查可见中剂量组雄性大鼠WBC明显低于对照组(p<0.01),但无剂量-反应关系。中、高剂量组雄性大鼠RBC和HGB均明显低于对照组(p<0.01);雌性大鼠的血常规检查未见明显改变。血清生化测定结果可见雄性大鼠中剂量组的BUN明显低于对照组(p<0.05),但无剂量-反应关系;中、高剂量组的TG明显低于对照组(p<0.05、p<0.01),高剂量组的GLU明显低于对照组(p<0.05),提示受检样品对雄性大鼠糖代谢和脂代谢有一定影响;雌性大鼠中、高剂量组的ALP明显低于对照组(p<0.05,p<0.01),高剂量的AST明显高于对照组(p<0.05)、GLU明显低于对照组(p<0.05),提示受检样品对雌性大鼠肝功能、糖代谢有一定影响。中剂量的CHE明显高于对照组(p<0.01),但无剂量-反应关系。尿常规检查:各剂量组与对照组比较,各项检测指标无明显变化。脏器检查和脏器重量系数测定:试验结束进行大体解剖,各剂量组动物大体解剖结果均未见异常。脏器重量系数测定结果表明,中、高剂量组雄性大鼠脑、肝、肾、睾丸重量系数明显高于对照组(p<0.05,p<0.01),高剂量组雌性大鼠脑、肝重量系数明显高于对照组(p<0.05,p<0.01)。病理组织学检查:高剂量组一只雌性大鼠肾小管管型;其它各脏器组织病理学检查未见明显病理改变。结论:除草啶原药对雄、雌性大鼠经口染毒剂量为215.0 mg/kg bw.d及以上时,对大鼠有毒性效应。除草啶原药对雄、雌性大鼠亚慢性经口毒性最大无作用剂量为107.5 mg/kg bw.d。除草啶原药对血脂代谢产生了一定影响。

Bromacil of the Original Observation of Drug Toxicity

To achieve the largest non-role of parameters for the safe production and chronic toxicity test to provide a reference dose,Subjects Bromacil were defined toxicity characteristics.We are based on GB15670-1995 "registered pesticide toxicology test methods"of its 90 d toxicity test.Results:The average weeks weight of the high-dose group male rats from the second weeks to the end of the experiment was significantly lower than that of the control group(p<0.05,p<0.01),the WBC of the middle and high dose group male rats was significantly lower than that of the control group(p<0.05),suggesting that subjects materials have some inhibitory effect on the WBC and the growth of male rats,the RBC and HGB of the high-dose group female rats was significantly lowerr than that of the control group(p<0.01),The PLT,TG and GLU of the middle-dose group male,female rats was significantly lower than that of the control group(p<0.01),prompted subjects materials have a certain influence on rat liver function and carbohydrate metabolism,organ weight results show that the kidney weight of the middle and high-dose group male rats and the high-dose group female rats was significantly higher than that of the control group(p<0.01,p<0.05),the liver weight of the middle and high dose group male and female rats was significantly higher than that of the control group(p<0.01,p<0.05),prompted the brain,the kidney,the liver and the spermary have been compensation function changes,the histopathological examination has not found organic changes and the dose group was no significant difference as compared with the control group.Conclusion:Bromacil male rats and female rats exposed to oral doses of 215 mg/kg bw.d and above,having the toxic effect on rats.Therefore,in this experiment,the largest no role dose of male and female was 107.50 mg/kg bw.d.Bromacil had an impacts on lipid and blood sugar metabolism.