| 温和气单胞菌单克隆抗体的制备及特性分析 |
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| 引用本文： | 黄钧,梁明龙,朱芸.温和气单胞菌单克隆抗体的制备及特性分析[J].淮阴师范学院学报(自然科学版),2006,5(1):74-77. |
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| 作者姓名： | 黄钧 梁明龙 朱芸 |
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| 作者单位： | 广西大学,动物科技学院,广西,南宁,530004 |
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| 摘 要： | 利用细胞融合技术建立了5株分泌抗温和气单胞菌CR79-1-1株单克隆抗体(McAb)的杂交瘤细胞系,通过特异性鉴定获得两株与参考菌株中的温和气单胞菌发生反应,但与嗜水气单胞菌以及鳗弧菌、爱德华氏菌、克鲁氏耶尔森菌、大肠杆菌、荧光假单胞菌均不反应的杂交瘤细胞系,分别命名为2G3和1A4.快速酶联免疫分析法(ELISA)测得2G3和1A4的亲和常数分别为1.72×108和5×108M-1;应用方阵配对实验证实,2株单抗针对特异性抗原上不同表位.
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| 关 键 词： | 温和气单胞菌 单克隆抗体 表位 酶联免疫吸附分析 |
| 文章编号： | 1670-6876（2006）01-0074-04 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005年9月28日 |
Preparation and Characteriaztion of Monoclonal Antibodies to Aeromonas Sobria |
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| HUANG Jun,LIANG Ming-long,ZHU Yun.Preparation and Characteriaztion of Monoclonal Antibodies to Aeromonas Sobria[J].Journal of Huaiyin Teachers College(Natrual Science Edition),2006,5(1):74-77. |
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| Authors: | HUANG Jun LIANG Ming-long ZHU Yun |
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| Abstract: | Five hybridoma cell lines(1A4,2C7,3G9,3D5 and 2G3)producing monoclonal antibodies(McAbs) against Aeromonas sobria CR79-1-1 strain were established by the fusion technique.The specificity analysis by indirect ELISA showed that 2G3 and 1A4 lines can react with A.sobria.None of them had a cross reaction with A.hydrophila,Vibrio anguillarum,Edwardsiella,Yersinia ruckeri,Escherichia coli and Pseudomonas fluorescens.Affinity constants of 2G3 and 1A4 are 1.72×10~8 and 5×10~8M ~(—) 1,respectively.Different epitopes of A.sobria were recognized by the two McAbs. |
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| Keywords: | Aeromonas sobria McAb epitope ELISA |
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