| 微量Se保护细胞膜和细胞内DNA免受~1O_2损伤的研究 |
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| 引用本文： | 廖宝凉,徐辉碧,邢军,伍跃辉.微量Se保护细胞膜和细胞内DNA免受~1O_2损伤的研究[J].华中科技大学学报(自然科学版),1991(1). |
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| 作者姓名： | 廖宝凉 徐辉碧 邢军 伍跃辉 |
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| 作者单位： | 华中理工大学化学系
(廖宝凉，徐辉碧，邢军)，华中理工大学化学系(伍跃辉) |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 本文利用亚甲蓝光敏化产生~1O_2,对~1O_2的荧光检测法做了研究;利用滤膜过滤法研究了硒化合物保护NIH小鼠肾细胞DNA免受~1O_2损伤的作用;利用~3H-TdR掺入法研究了Na_2SeO_3对~1O_2抑制Wistar大鼠骨髓细胞DNA合成的作用;利用TBA法检测细胞脂质过氧化作用,观察到Na_2SeO_3抑制~1O_2诱导的细胞脂质过氧化的作用.Na_2SeO_3起保护作用的最适宜浓度范围为0.01～1.00μmol/L,高浓度的Na_2SeO_3表现出毒性作用.
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| 关 键 词： | 微量元素 硒 DNA 单线态氧 保护细胞 毒性 |
A Study on the Protection of Cell Membrane and DNA with Trace Selenium against Damage by Singlet Oxygen |
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| Liao Baoiiang Xu Huibi Xing Jun Wu Yuehui.A Study on the Protection of Cell Membrane and DNA with Trace Selenium against Damage by Singlet Oxygen[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,1991(1). |
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| Authors: | Liao Baoiiang Xu Huibi Xing Jun Wu Yuehui |
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| Institution: | Liao Baoiiang Xu Huibi Xing Jun Wu Yuehui |
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| Abstract: | Singlet oxygen (~1O_2) has been generated by photosensitive reaction of methylene blue. The detection of singlet oxygen by fluorometry is studied. The protection against damage of DNA in renal cells of young rats by singlet oxygen with selenium compounds (Na2SeO3 and ebselen) has been investigated with the Kohn filtering method. The effect of Na2SeO3 on the inhibition of singlet oxygen to DNA synthesis of a rat's marrow cells has been observed using 3H-TdR mixing method. The dtermination of MDA by the TBA method shows that Na2SeO3 inhibits the lipid peroxidation of cells induced by singlet oxygen. The proper concentration of Na2SeO3 for protecting DNA is about 0.10umol/L. A higher concentration exhibits toxicity which may be due to the generation of active oxygen. |
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| Keywords: | Trace element Selenium DNA Singlet oxygen Cell protection Toxicity |
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