长白山林蛙输卵管糖蛋白的分离鉴定

以长白山林蛙为研究对象, 对其输卵管糖蛋白进行提取分离及鉴定. 长白山林蛙输卵管冲洗液经饱和硫酸铵沉淀, DEAE-Cellulose 52离子交换柱, Sephadex G-100凝胶柱层析纯化, 得到蛋白质和糖的重合峰, 经透析、 冷冻干燥得长白山林蛙输卵管糖蛋白纯品OGP-Ⅰ. SDS PAGE电泳实验结果表明, OGP-Ⅰ是分子量为116 000的均一带. 对其分别进行蛋白和糖染色, 同一位置都出现单一条带； 紫外全扫描OGP-Ⅰ有多糖特征峰和蛋白吸收峰, 表明OGP-Ⅰ是糖蛋白纯品.

Separation and Identification of Rana c Changbaishanensis Oviduct Glycoprotein

Rana chensinensis Changbaishanensis oviduct glycoprotein was separated and identified in the test. Saturated ammonium sulfate was uesd to pre
cipitate Rana c Changbaishanensis oviduct glycoprotein(the optimization saturated degree of saturated ammonium sulfate is 70%), followed by DEAE Cellulose 52 ion exchange chromatography initial purification, then Sephadex G 100 further purification.To collect the overlap peak of protein and sugar. It’s the Rana c. Changbaishanensis oviduct glycoprotein sterling(OGP-Ⅰ) after lyophillization. The result of SDS PAG electrophoresis indicates OGP-Ⅰ is only a colored zone. The molecular weight of glycoprotein was 116 000. The result of SDS PAG electrophoresis combined with coomassie brilliant blue staining and shiff staining provs it is glycoprotein, OGP-Ⅰ is only a colored zone at the same local. The ultra violet scanning of OGP-Ⅰ indicates that it exhibits polysaccharide characteristic absorption band and protein absorption band. Therefore
, these can further indicate that OGP-Ⅰ is glycoprotein.