| 小麦谷氨酰胺合成酶(GS)的器官分布及光照对GS基因表达的影响 |
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| 引用本文： | 印莉萍,刘祥林,吴晓强,李欣,邱泽生,张承谦,赵微平.小麦谷氨酰胺合成酶(GS)的器官分布及光照对GS基因表达的影响[J].首都师范大学学报(自然科学版),1993(4). |
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| 作者姓名： | 印莉萍 刘祥林 吴晓强 李欣 邱泽生 张承谦 赵微平 |
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| 作者单位： | 首都师范大学生物系
(印莉萍，刘祥林，吴晓强，李欣，邱泽生，张承谦)，首都师范大学生物系(赵微平) |
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| 摘 要： | 用组织印迹法、Northern分子杂交、DEAE-纤维素柱层析技术、酶活性测定以及抑制剂等研究了小麦幼苗各器官谷氨酰胺合成酶(GS)活性的变化和光对GS活性及GS-mRNA的影响。结果表明:小麦幼苗各器官的GS活性和特性是不一样的。PPT对绿叶中GS的抑制高于根部,对叶绿体谷氨酰胺合成酶(GS2)的抑制强于细胞质谷氨酰胺合成酶(GSI)。组织印迹杂交测定了小麦汁液GS-mRNA的器官组织专一性基因的表达。光对总RNA和GS-mRNA有明显的诱导作用。
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| 关 键 词： | 谷氨酰胺合成酶 基因表达 光照 小麦 |
Distribution of Glutamine Synthetase (GS) in Wheat and Influence of Light on the Expression of GS Gene |
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| Yin Liping,Liu Xianglin,Wu Xiaoqiang,Li Xin,Qiu Zesheng,Zhang Chengqian,Zhao Weiping.Distribution of Glutamine Synthetase (GS) in Wheat and Influence of Light on the Expression of GS Gene[J].Journal of Capital Normal University(Natural Science Edition),1993(4). |
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| Authors: | Yin Liping Liu Xianglin Wu Xiaoqiang Li Xin Qiu Zesheng Zhang Chengqian Zhao Weiping |
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| Institution: | Yin Liping;Liu Xianglin;Wu Xiaoqiang;Li Xin;Qiu Zesheng;Zhang Chengqian;Zhao Weiping Department of Biology. Capital Normal University |
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| Abstract: | The activityes of GS from the different organs of wheat seedlings and influence of light on GS and GS-mRNA have been studied by the employment of TPH, Northern blot,DEAE-celluose columns chromatography, enzyme activity assay and inhibitor. The resultsindicated that the activity of GS among various organs of wheat seedlings was quite differ-ent. The activity of GS isolated from green leaves was more inhibited by PPT than thatfrom roots, and moreover, the activity of chlorplast glutamine synthetase (GS2) was in-hibited by PPT more stronger than that of cytoplasm glutamine synthetase (GS1). Thetissue specific gene expression of the GS-mRNA in wheat seedlings was analyzed by usingTPH. The results of Northern blotting indicated that total RNA and GS-mRNA was evidently induced by light. |
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| Keywords: | glutamine synthetase gene expression light wheat |
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