| 一株无机磷细菌的分离、鉴定及其溶磷能力分析 |
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| 引用本文： | 王继雯,谢宝恩,甄静,刘莹莹,李冠杰. 一株无机磷细菌的分离、鉴定及其溶磷能力分析[J]. 河南科学, 2011, 29(3): 293-296 |
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| 作者姓名： | 王继雯 谢宝恩 甄静 刘莹莹 李冠杰 |
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| 作者单位： | 1. 河南省微生物工程重点实验室,郑州,450008
2. 河南省科学院生物研究所有限责任公司,郑州,450008 |
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| 基金项目： | 河南省重大公益科研招标项目 |
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| 摘 要： | 从根系土壤分离纯化出降解无机磷的细菌8株,通过平板法进行初筛,根据水解圈直径和菌落直径比值大小筛选到水解圈直径和菌落直径比值较大的菌株3株,进一步通过液体摇瓶培养复筛出I株分解无机磷能力最强的菌株PC-2,该菌株经形态特征,生理生化特性16 S rDNA序列测定等一系列试验鉴定为地衣芽孢杆菌(Bacillus lich...
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| 关 键 词： | 无机磷细菌 分离 鉴定 溶磷能力 pH |
Screening and Identification of a Inorganic Phosphorus-Dissolving Bacteria from Soil and Its Analysis on the Phosphate Solubilization |
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| Wang Jiwen,Xie Baoen,Zhen Jing,Liu Yingying,Li Guanjie. Screening and Identification of a Inorganic Phosphorus-Dissolving Bacteria from Soil and Its Analysis on the Phosphate Solubilization[J]. Henan Science, 2011, 29(3): 293-296 |
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| Authors: | Wang Jiwen Xie Baoen Zhen Jing Liu Yingying Li Guanjie |
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| Affiliation: | 1.Key Laboratory of Microbial Engineering of Henan,Zhengzhou 450008,China; 2.Institute of Biology at Henan Academy of Sciences,Zhengzhou 450008,China) |
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| Abstract: | Eight inorganic phosphorus-dissolving bacterium stains were obtained from soils of different rhizospheres.Through the plate method for the first screening,three bacterial strains with the larger ratio of hydrolysis circle diameter to colony diameter were got.Of these strains,one the best strain(strain PC-2),which shows high inorganic phosphorus-degradation ability up to 57.5 mg/L.It is identified as bacillus licheniformis by its morphological and physiological characteristics as well as by analysis of the gene encoding the 16S rRNA.And it has been discovered that there are significant relevance between solubilizing phosphorus concentration and pH of fermented fluid in different time,but it is not proportional to the relevant cases. |
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| Keywords: | pH |
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