| 农杆菌介导牧草蔗42遗传转化体系的建立 |
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| 引用本文： | 翁频,陈亮,陈睦传,卢川北,洪月云.农杆菌介导牧草蔗42遗传转化体系的建立[J].厦门大学学报(自然科学版),2002,41(5):536-540. |
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| 作者姓名： | 翁频 陈亮 陈睦传 卢川北 洪月云 |
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| 作者单位： | 1. 厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建,厦门,361005
2. 福建农业科学院甘蔗研究所,福建,漳州,363005 |
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| 摘 要： | 将拟南芥叶片衰老特异启动子sag12与异戊烯基转移酶基因ipt构成的嵌合基因经农杆菌介导转入牧草蔗42中，建立了农杆菌转化牧草蔗42的遗传转化系统，已经获得转基因再生植株，PCR及Southern杂交检测证实ipt基因确实转移到牧草蔗42中，报告基因GUS在再生植株中也得到表达。
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| 关 键 词： | 遗传转化体系 牧草蔗42 ipt基因 农杆菌 异戊烯基转移酶基因 转基因植株 |
| 文章编号： | 0438-0479(2002)05-0536-05 |
| 修稿时间： | 2002年5月20日 |
Study on Transformation of Sugarcane-grass 42 Mediated by Agrobacterium |
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| WENG Pin ,CHEN Liang ,CHEN Mu-zhuan ,LU Chuan-bei ,HONG Yue-yun.Study on Transformation of Sugarcane-grass 42 Mediated by Agrobacterium[J].Journal of Xiamen University(Natural Science),2002,41(5):536-540. |
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| Authors: | WENG Pin CHEN Liang CHEN Mu-zhuan LU Chuan-bei HONG Yue-yun |
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| Institution: | WENG Pin 1,CHEN Liang 1,CHEN Mu-zhuan 1,LU Chuan-bei 2,HONG Yue-yun 2 |
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| Abstract: | Sugarcane-grass 42 is a newly formed breed of PT43-52 and Co419 in subtropic area. We introduced chimeric gene containing promoter sag12 and ipt gene to sugarcane-grass 42 mediated by Agorbacterium. We have established an optimum protocol of transformation and already obtained the seedling regenerated from transformants. Factors such as vacuuming or not, inoculating time, AS concentration et.al were also studied. PCR and Southern blotting of seedlings revealed that the ipt gene has transfered to sugarcane-grass42 genomes. |
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| Keywords: | sugarcane-grass 42 transformation ipt gene Agrobacterium |
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