| 表皮生长因子效应评价细胞模型的建立 |
| |
| 引用本文： | 陈亮,穆蕊,甄诚,高彦飞,李腾,于鸣,巩伟丽,李爱玲.表皮生长因子效应评价细胞模型的建立[J].科学技术与工程,2011,11(17):3903-3905,3918. |
| |
| 作者姓名： | 陈亮 穆蕊 甄诚 高彦飞 李腾 于鸣 巩伟丽 李爱玲 |
| |
| 作者单位： | 1. 国家生物医学分析中心,北京,100850
2. 军事医学科学院基础医学研究所细胞免疫学教研室,北京,100850 |
| |
| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
| |
| 摘 要： | 为了筛选表皮生长因子(EGF)信号通路的未知调控分子,利用人正常乳腺上皮细胞系MCF10A建立了EGF效应评价模型,包括细胞增殖、迁移以及细胞周期的进程等。结果显示,EGF能有效促进MCF10A细胞的增殖。饥饿同步化后,EGF能促进细胞从G1期进入S期,开始DNA的合成。此外,Transwell实验显示EGF能明显促进MCF10A细胞的迁移。
|
| 关 键 词： | 表皮生长因子 MCF10A 细胞周期 |
| 收稿时间： | 3/7/2011 1:12:17 PM |
| 修稿时间： | 2011/3/10 0:00:00 |
Establishment of the Cell Model used for Epidermal Growth Factor Effect Evaluation |
| |
| CHEN Liang,MU Rui,ZHEN Cheng,GAO Yan-fei,LI Teng,YU Ming,GONG Wei-li,LI Ai-ling.Establishment of the Cell Model used for Epidermal Growth Factor Effect Evaluation[J].Science Technology and Engineering,2011,11(17):3903-3905,3918. |
| |
| Authors: | CHEN Liang MU Rui ZHEN Cheng GAO Yan-fei LI Teng YU Ming GONG Wei-li LI Ai-ling |
| |
| Institution: | CHEN Liang~1,MU Rui~1,ZHEN Cheng~1,GAO Yan-fei~1,LI Teng~1,YU Ming~2,GONG Wei-li~1,LI Ai-ling~(1*) (National Center of Biomedical Analasis 1,Beijing 100850,P.R.China,Department of Molecular Immunology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences 2,P.R.China) |
| |
| Abstract: | To screen the unknown regulator of the Epidemal Growth Factor (EGF) signaling pathway, we set up a cell model using MCF10A, a human normal mammary epidermal cell line, for evaluation of EGF effect, including cell proliferation, cell migration and cell cycle progression. The results showed the significant increase in MCF10A cell proliferation with EGF treatment. After cell synchronized by serum starvation, cells proceeded from G1 phase to S phase and initiated DNA synthesis with EGF treatment. In addition, transwell assay showed that EGF obviously promoted MCF10A cell migration. |
| |
| Keywords: | epidermal growth factor MCF10A cell cycle |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《科学技术与工程》浏览原始摘要信息 |
| 点击此处可从《科学技术与工程》下载免费的PDF全文 |
|
