Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10 |
| |
Authors: | Angela Dittmer Kristina Hohlfeld Jana Lützkendorf Lutz P. Müller Jürgen Dittmer |
| |
Affiliation: | 1. Klinik für Gyn?kologie, Universit?t Halle, Halle (Saale), Germany 2. Zentrum für Innere Medizin, Klinik für Innere Medizin IV, Universit?t Halle, Halle (Saale), Germany
|
| |
Abstract: | Mesenchymal stem cells (MSCs) have been shown to communicate with tumor cells. We analyzed the effect of human MSCs (hMSCs) on breast cancer cells in three-dimensional cultures. By using GFP expression and immunohistochemistry, we show that hMSCs invade 3D breast cancer cell aggregates. hMSCs caused breast cancer spheroids to become disorganized which was accompanied by a disruption of cell–cell adhesion, E-cadherin cleavage, and nuclear translocation of E-cadherin, but not by epithelial/mesenchymal transition or by an increase in ERK1/2 activity. In addition, hMSCs enhanced the motility of breast cancer cells. Inhibition of ADAM10 (a disintegrin and metalloprotease 10), known to cleave E-cadherin, prevented both hMSC-mediated E-cadherin cleavage and enhanced migration. Our data suggest that hMSCs interfere with cell–cell adhesion and enhance migration of breast cancer cells by activating ADAM10. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|