| 模拟酶用于测定葡萄糖及免疫分析的研究 |
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| 引用本文： | 姚凤姬,王林涛,慈云祥.模拟酶用于测定葡萄糖及免疫分析的研究[J].北京大学学报(自然科学版),1999,35(4):437-440. |
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| 作者姓名： | 姚凤姬 王林涛 慈云祥 |
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| 作者单位： | 北京大学化学与分子工程学院，北京，100871 |
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| 摘 要： | 研究锰-四磺基苯卟啉(Mn-TPPS4)络合物催化过氧化氢与邻苯二胺反应的特性，并与辣根过氧化物酶比较。与葡萄糖氧化酶的酶促反应偶联，测定血清中的葡萄糖，与试剂盒测定结果吻合。用两种方法将Mn-TPPS4标记到牛血清白蛋白上，其免疫活性和催化活性均较好。
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| 关 键 词： | 过氧化物模拟酶 催化显色 葡萄糖的测定 标记试剂 |
| 收稿时间： | 1998-04-21 |
Studies on the Application of Mimetic Peroxidase for the Determination of Glucose and Immunoassay |
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| YAO Fengji,WANG Lintao,CI Yunxiang.Studies on the Application of Mimetic Peroxidase for the Determination of Glucose and Immunoassay[J].Acta Scientiarum Naturalium Universitatis Pekinensis,1999,35(4):437-440. |
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| Authors: | YAO Fengji WANG Lintao CI Yunxiang |
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| Institution: | College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 |
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| Abstract: | The catalytic characteristics of Mn TPPS 4 as mimesis of peroxidase on chromog enic reaction of hydrogen peroxide with o phenylenediamine have been studie d and characteristics of Mn TPPS 4 were compared with those of horseradish per oxidase.The glucose in human serum has been determined by the proposed reaction combining with catalytic reaction of glucose oxidase with satisfactory results.L abeling of bovine serum albumin(BSA) with Mn TPPS 4 was carried.Results in stu died active of the labeling reagent shown promising for an immunoassay. |
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| Keywords: | mimetic Peroxidase determination of glucose catalytic reaction labeled reagen t |
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