两个封闭群昆明小鼠行为学差异和试验方法信度分析

摘要: 目的 探讨两个封闭群昆明小鼠的行为学差异并评估行为学试验方法检测昆明小鼠行为的可信度。方法 应用小鼠行为学试验检测两个不同封闭群昆明小鼠的行为差异并做试验方法的复测信度检验。两个封闭群昆明 小鼠各取 20 只雄性小鼠分别记为 A 组、B 组，两组小鼠经过适应性饲养 3 d 后进行首次试验，记录体质量( 之后每 天记录体质量并绘制体质量增长趋势图) ，做衣架试验、网屏试验、蔗糖偏嗜度试验、旷场试验和强迫游泳试验等。 相隔 7d 进行复测试验。用 SPSS17. 0 软件处理数据并检验各行为学方法的复测信度。结果 行为学差异检测中 A 组、B 组首测昆明小鼠体质量、蔗糖偏嗜度、旷场试验水平运动、总分、网屏试验各项结果差异具有统计学意义( P ＜ 0. 05) ; 复测试验中体质量、蔗糖偏嗜度、网屏试验差异具有统计学意义( P ＜ 0. 05) ; 复测信度试验中粪便颗粒试验 为不可信( 信度系数为负值) ，衣架试验和旷场试验中垂直运动记分可信度较差，其它试验相关性均为可信( 体质量 为高度可信) 。结论 A、B 两组封闭群昆明小鼠体质量增长速度、蔗糖偏嗜度和网屏试验存在差异; 行为学检测方 法中信度评级为体质量 ＞ 强迫游泳 ＞ 蔗糖偏嗜度 ＞ 水平运动 ＞ 总分 ＞ 网屏试验 ＞ 衣架试验 ＞ 垂直运动。

The Differences of Ethology and Retest Reliability Analysis in Two Closed Group of Kunming Mice

Objective To inspect the behavioral differences and the retest reliability of methods in two closed group of Kunming mice （KM）. Method The behavioral differences and the retest reliability of methods in two closed group KM mice were detected by ethological methods. Different closed groups were divided into A, B two groups of 20 male mice. The data of weight, coat hanger, inverted screen, sucrose preference, open field test and forced swimming test were recorded to inspect the behavioral differences and the retest reliability of methods by SPSS17.0 software after 3d adaptive feeding. The same tests were repeated after 7 d. Result The items of weight, sucrose preference, the horizontal movement of open field test , score, inverted screen were significantly different （P 〈 0.05） in the statistics at the first experiment. All items of weight, sucrose preference, inverted screen were significantly different （P 〈 0.05） in the statistics in the retest reliability experiment. Fecal pellets test was not credible （reliability coefficient was negative） , hanger test and open-field test in the vertical motion score were poor credibility,but other tests are credible （weight of highly credible）. Conclusion The behavioral differences exist between A and B groups KM mice. The results of reliability ratings are weight 〉 forced swimming test 〉 sucrose preference 〉 horizontal movement 〉 score 〉 inverted screen 〉 coat hanger 〉 vertical movement.