| 抗牙周病厌氧菌（Bg）单链抗体基因的构建与表达 |
| |
| 引用本文： | 魏云林,龚春梅,张玲琪,傅家忠,路虹,林一南,曹丽华,吉耀红.抗牙周病厌氧菌（Bg）单链抗体基因的构建与表达[J].云南大学学报(自然科学版),1995(3). |
| |
| 作者姓名： | 魏云林 龚春梅 张玲琪 傅家忠 路虹 林一南 曹丽华 吉耀红 |
| |
| 作者单位： | 中国医学科学院医学生物学研究所，云南大学生物系，云南省红十字会医院 |
| |
| 摘 要： | 从牙周病厌氧菌（Ｂａｃｔｅｒｏｉｄｅｓｇｉｎｇｉｖａｌｉｓ）单克隆抗体的杂交瘤细胞株出发，通过基因工程方法──ＰＣＲ技术，调出其抗体的重链和轻链基因可变区序列，体外重组后得到单链抗体ＳｃＦｖ（ｓｉｎｇｌｅｃｈａｉｎｆｒａｇｍｅｎｔｖａｒｉａｂｌｅ）片段，克隆到了ｐＣＡＮＴＡＢ５载体上，在ＥｓｃｈｅｒｉｃｈｉａｃｏｌｉＴＧｌ中得到了成功表达。
|
| 关 键 词： | 牙周病厌氧菌（Bg），PCR技术，单链抗体（ScFv），克隆，表达 |
Anti-Bacteroides gingivalis Single Chain Fragment Variable Cloned and Expressed in Escherichia coli TG1 |
| |
| Wei Yunlin,Gong Chunmei,Zhang Linqi,Fu Jiazhong Lu Hong,Lin Yinan,Cao Lihua,Ji Yaohong.Anti-Bacteroides gingivalis Single Chain Fragment Variable Cloned and Expressed in Escherichia coli TG1[J].Journal of Yunnan University(Natural Sciences),1995(3). |
| |
| Authors: | Wei Yunlin Gong Chunmei Zhang Linqi Fu Jiazhong Lu Hong Lin Yinan Cao Lihua Ji Yaohong |
| |
| Abstract: | The hybridoma producting Anti-Bacteroldes gingivalis(Bg) antibody wasused as a begining strain. With PCR technology, the antibody heavy and light chain vari-able domains have been got, and was comprised them linked into a single chain bv a flexi-ble peptides which termed ScFv(single chain fragment of variable).The ScFv was clonedin vector pCANTAB5 and expressed in E, coli TG 1. The product of ScFv expressed ap-peared biological activity detected by ELISA method. |
| |
| Keywords: | Bacteroldes gingivalis(Bg) PCR ScFv(single chain fragment of varia-ble) clone express |
| 本文献已被 CNKI 等数据库收录！ |
