Tagging major quantitative trait loci for sheath blight resistance in a rice variety, Jasmine 85 |
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Authors: | Xuebiao Pan Junhuang Zou Zongxiang Chen Jufei Lu Hengxiu Yu Haitao Li Zibin Wang Xingyuan Pan M C Rush Lihuang Zhu |
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Institution: | (1) Agricultural College, Yangzhou University, 225009 Yangzkou, China;(2) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(3) Louisiana State University, 70803 Baton Rouge, LA, USA |
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Abstract: | This study was conducted with a clonal F2 population of rice from a cross between Jasmine 85, a resistant variety, and Lemont, a susceptible cultivar. The rice plants
belonging to each F2 clone were divided into two plots, which were put in two replicates, respectively. Clonal parents were tested as controls.
The plants were inoculated by short toothpicks incubated with RH-9, a virulent isolate of the pathogenic fungus,Rhizoctonia solani, which causes rice sheath blight. The extreme resistant and susceptible clonal lines were selected for construction of resistant
and susceptible DNA pools, respectively. A total of 94 polymorphic markers evenly distributed on 12 rice chromosomes were
used for bulked segregant analysis, three positive ones were found polymorphic between the two DNA pools, and three major
QTLs for sheath blight resistance, Rh-2, Rh-3 and Rh-7, were identified. The three major QTLs were located on chromosomes
2, 3 and 7, and could explain 14.4%, 26.1% and 22.2% of the phenotypic variation. |
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Keywords: | rice sheath blight major QTLs RFLP |
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