| 微量超氧化物歧化酶邻苯三酚/Vc测活法 |
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| 引用本文： | 王会文,刘建荣,静天玉,赵晓瑜.微量超氧化物歧化酶邻苯三酚/Vc测活法[J].河北大学学报(自然科学版),2000,20(1):61-62,82. |
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| 作者姓名： | 王会文 刘建荣 静天玉 赵晓瑜 |
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| 作者单位： | 河北大学,生物工程研究所,河北,保定,071002 |
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| 摘 要： | 在Vc终止剂法的基础上 ,邻苯三酚的加入量由终浓度2mmol/L降低为0.05mmol/L ,并提高反应溶液的 pH值至8.6 ,从而提高了SOD活力测定的灵敏度。使用本方法在SOD样品最大加入量1mL时 ,最低可检测到3.3×10-8 mol/(s·mL)SOD ,比Vc终止剂法灵敏度提高4倍。
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| 关 键 词： | 超氧化物歧化酶(SOD) 邻苯三酚 L-抗坏血酸(Vc) |
| 文章编号： | 1000-1565(2000)01-0061-02 |
| 修稿时间： | 1999-05-17 |
A Microassay of Superoxide Dismutase(SOD) Based on Pyrogallol/Vc Method |
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| WANG Hui-wen,LIU Jian-rong,JING Tian-yu,ZHAO Xiao-yu.A Microassay of Superoxide Dismutase(SOD) Based on Pyrogallol/Vc Method[J].Journal of Hebei University (Natural Science Edition),2000,20(1):61-62,82. |
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| Authors: | WANG Hui-wen LIU Jian-rong JING Tian-yu ZHAO Xiao-yu |
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| Institution: | WANG Hui-wen
,LIU Jian-rong
,JING Tian-yu
,ZHAO Xiao-yu
(Institute of Biotechnology,Hebei University,Baoding 071002,China) |
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| Abstract: | The sensitivity of pyrogallol/Vc method for SOD assay could be increased 4 times over the original one by reducing pyrogallol amount down to 0.05 mmol/L and increasing pH volume up to 8.6. When 1 mL sample was added to the reaction system, 3.3×10-8 mol/(s·mL) SOD can be detected. |
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| Keywords: | superoxide dismutase(SOD) pyrogallol L-ascorbic acid(Vc) |
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