基于灵芝双向固体发酵雷公藤减毒持效的研究

传统中药雷公藤具有抗炎和免疫抑制的作用，但因毒性强而极大地限制了其临床应用。以灵芝为药用真菌，采用双向固体发酵技术发酵雷公藤，并对发酵过程中得到的灵雷菌质进行抗炎活性和肝毒性评价。结果表明，接种量10 mL/100 mL、发酵30天得到的灵雷菌质（N2-G30）在3.75 μg/mL的质量浓度下表现出减毒持效的作用，相比于雷公藤，可抑制脂多糖诱导的小鼠RAW264.7细胞释放促炎因子TNF-α和IL-6，并且可减轻对人正常肝细胞L02增殖的抑制作用。通过液相色谱-质谱联用（LC-MS）分析雷公藤发酵前后主要成分的含量变化，结果显示，与发酵前相比，发酵后雷公藤甲素、雷公藤红素、雷酚内酯的含量增加，雷公藤内酯甲的含量减少，这些成分的含量变化与N2-G30的减毒持效作用有关。

Detoxification and sustained effects of Tripterygium wilfordii based on Ganoderma lucidum bi-directional solid fermentation

The traditional Chinese medicine Tripterygium wilfordii has anti-inflammatory and immunosuppressive effects, but its clinical application is greatly limited due to its strong toxicity. Ganoderma lucidum was used as a medicinal fungus to ferment Tripterygium wilfordii by bi-directional solid fermentation. The anti-inflammatory activity and hepatotoxicity of the fungal fermentation product were evaluated. The results showed that the product (N2-G30) obtained at an inoculation amount of 10 mL/100 mL and after 30 days of fermentation at a mass concentration of 3.75 μg/mL afforded a detoxification and sustained effect. Compared with Tripterygium wilfordii, the product can inhibit the release of pro-inflammatory cytokines TNF-α and IL-6 from RAW264.7 cells induced by lipopolysaccharide and reduce the inhibition of the proliferation of human normal liver cells L02. The main components of Tripterygium wilfordii before and after fermentation were analyzed by liquid chromatography-mass spectrometry (LC-MS). The results showed that the contents of triptolide, celastrol and triptophenolide increased and the contents of wilforlide A decreased after fermentation. The changes in the concentrations of these components are reflected in the detoxification and sustained effect of N2-G30.