利用加强型绿色荧光蛋白标记黄色单胞菌的研究

通过连接T载体与来自质粒pSEBG上的egfp基因而构建成质粒pZTE,并将质粒pZTE的egfp基因插入到自杀质粒pTnMod-OGm转座子上的多克隆位点Kpn I和Sal I中构建成质粒pTE-OGm,最后pTE-OGm的egfp基因转座子元件通过转座作用插入到受体菌黄色单胞菌L1的染色体中,从而使黄色单胞菌L1获得较稳定的绿色荧光标记,为进一步在MBR反应器中更好地示踪黄色单胞菌L1的生长和迁移情况奠定了基础。

Labeling Gram-negative Bacteria Using Enhanced Green Fluorescent Protein

By connecting the T vector and the egfp gene from plasmid pSEBG to construct the plasmid pZTE, then the egfp gene of the plasmid pZTE was inserted into multiple cloning sites of Kpn I and Sal I in the suicide vector pTnMod-OGm to construct the plasmid pTE-OGm. Last, the transposon elements of egfp gene in pTE-OGm translocated into the chromosome of the strain Xanthomonas Campestris L1, so that the strain Xanthomonas Campestris L1 obtain stable green fluorescent marker, for better to trace the migration and foundation of Xanthomonas Campestris L1 in MBR reactor for further.