| 利用柑橘茎段诱导不定芽改进微芽嫁接技术的研究 |
| |
| 引用本文： | 陈泽雄,刘奕清,娄娟.利用柑橘茎段诱导不定芽改进微芽嫁接技术的研究[J].西南师范大学学报(自然科学版),2007,32(1):57-61. |
| |
| 作者姓名： | 陈泽雄 刘奕清 娄娟 |
| |
| 作者单位： | 重庆文理学院,生命科学系,重庆,永川,402168 |
| |
| 基金项目： | 重庆文理学院校级课题资助项目（Z2005 SK 33） |
| |
| 摘 要： | 试验以成年态纽荷尔脐橙茎段为外植体，诱导产生不定芽改进微芽嫁接成活率与脱毒率．结果表明：半木质化茎段作为培养外植体污染率最低，不定芽诱导的最佳培养基为MT＋＋6-BA1．0mg／L＋NAA0．2mg／L＋GA30．2mg／L．将诱导的嫩芽进行微芽嫁接，成活率超过80．8％，对照成活率为61．3％，聚合酶链式反应（PCR）检测脱毒率达100％．
|
| 关 键 词： | 茎段 不定芽 微芽嫁接 脱毒率 |
| 文章编号： | 1000-5471（2007）01-0057-05 |
| 修稿时间： | 2006-12-04 |
Improvement of Shoot-Tip Micrografting Through Adventitious Bud Induction from Citrus Stem Explants |
| |
| CHEN Ze-xiong,LIU Yi-qing,LOU Juan.Improvement of Shoot-Tip Micrografting Through Adventitious Bud Induction from Citrus Stem Explants[J].Journal of Southwest China Normal University(Natural Science),2007,32(1):57-61. |
| |
| Authors: | CHEN Ze-xiong LIU Yi-qing LOU Juan |
| |
| Institution: | Department of Lile Science, Chongqing University of Arts and Science, gongchuan, Chongqing 402168, China |
| |
| Abstract: | Adventitious buds were induced with mature stem segments of Newhall navel orangeCitrus sinensis(L_*)Osbeck]as explants in an in vitro culture experiment to improve the survival rate and virus elimination rate in shoot-tip micro-grafting.Contamination rate was the lowest when half lignified stem segments were used as explants.MT++ 6-BA 1.0 mg/L+NAA 0.2 mg/L+GA_3 0.2 mg/L was the best medium for adventitious bud induction.More than 80.8% of the regenerated shoots survived when used in shoot-tip micro-grafting,in contrast to 61.3% for the control.PCR test showed that 100% of the grafted plants were virus free. |
| |
| Keywords: | stem segment adventitious bud shoot tip micro-grafting virus elimination rate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西南师范大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《西南师范大学学报(自然科学版)》下载免费的PDF全文 |
