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洱海下游肠道致病菌噬菌体分离与鉴定
引用本文:李洁,余泽英,赵宗美,颜怡君,马志刚,刘奇. 洱海下游肠道致病菌噬菌体分离与鉴定[J]. 大理学院学报:综合版, 2011, 0(12): 97-98
作者姓名:李洁  余泽英  赵宗美  颜怡君  马志刚  刘奇
作者单位:大理学院基础医学院
基金项目:大理学院大学生科研基金资助项目(KYSX2010-051)
摘    要:目的:分离洱海下游水体中的肠道致病菌噬菌体,了解肠道致病菌污染情况。方法:通过噬菌斑法从洱海下游水体中分离和鉴定各种肠道致病菌噬菌体。结果:成功分离出了痢疾杆菌噬菌体、伤寒杆菌噬菌体、乙型副伤寒杆菌噬菌体、大肠杆菌噬菌体4种肠道致病菌噬菌体。结论:洱海下游水体存在痢疾杆菌、伤寒杆菌、乙型副伤寒杆菌、大肠杆菌污染,提示需进一步加强大理周边生活污水排放的治理工作。

关 键 词:肠道致病菌  洱海  噬菌体

Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake
LI Jie,YU Zeying,ZHAO Zongmei,YAN Yijun,MA Zhigang,LIU Qi. Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake[J]. Journal of Dali University, 2011, 0(12): 97-98
Authors:LI Jie  YU Zeying  ZHAO Zongmei  YAN Yijun  MA Zhigang  LIU Qi
Affiliation:(School of Basic Medicine,Dali University,Dali,Yunnan 671000,China)
Abstract:Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and identification phage of intestinal bacteria.Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake.Results: Bacteriophages specific to dysentery bacillus,typhoid bacillus,paratyphoid B bacteria,E.coli were successfully isolated.Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus,typhoid bacillus,paratyphoid B bacteria and E.coli.This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.
Keywords:intestinal pathogen  Erhai Lake  phage
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