| 羚牛细胞色素b基因序列分析和系统进化研究 |
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| 引用本文： | 蒙世杰,宿兵,等.羚牛细胞色素b基因序列分析和系统进化研究[J].西北大学学报,2001,31(4):347-350,354. |
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| 作者姓名： | 蒙世杰 宿兵 |
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| 作者单位： | [1]西北大学生命科学学院，陕西西安710069 [2]中国科学院昆明动物研究所，云南昆明650233 |
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| 基金项目： | 中国科学院核心资助项目(97-04-20) |
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| 摘 要： | 应用聚合酶链式反应（PCR）分别扩增了羚牛、绵羊、山羊、黄牛细胞色素b基因，并对其全序列（1140bp）进行了测定。其中羚牛细胞色素b基因序列属首次报道。通过对8种偶蹄类动物细胞色素b基因序列差异分析和基于序列差异所构建的分子系统树，发现羚牛与羊亚科的动物亲缘关系最近，与其他动物亲缘关系较远。表明将羚牛放入羊亚科较为合理，序列差异分析还表明羚羊约在距今500万年前（上新世）从牛类动物中分化出来，牛类分化时间约在600万年前的中新世（Miocene）。
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| 关 键 词： | 羚牛 偶蹄目 细胞色素b基因 DNA序列分析 系统进化 羊亚科 分类 |
| 文章编号： | 1000-274(2001)04-0347-04 |
Analysis of cytochrome b gene of takim and its phylogeny |
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| MENG Shi jie ,WANG Jing ,LIU Pei ,SU Bing ,ZHANG Ya ping.Analysis of cytochrome b gene of takim and its phylogeny[J].Journal of Northwest University(Natural Science Edition),2001,31(4):347-350,354. |
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| Authors: | MENG Shi jie WANG Jing LIU Pei SU Bing ZHANG Ya ping |
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| Institution: | MENG Shi jie 1,WANG Jing 1,LIU Pei 1,SU Bing 2,ZHANG Ya ping 2 |
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| Abstract: | The 1 140 bp cytochrome b gene were amplified and sequenced from takin, sheep, goat, bovine. The sequence of takin was first reported. By analyzing the sequence divergence of 8 species of Artiodactyla and the phylogenetic tree based on the divergence, it was found that the genetic relationship between takin and the species of Caprine is closer than that between takin and the other related animals. So it is reasonable to place takin into Caprinae. In addition, it showed that takin diverged from Bovidae before 5 Mya and Bovidae diverged before 6 Mya. |
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| Keywords: | takin artiodactyla cytochrome b gene sequence analysis phylogeny |
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