| 缓冲液成分对固相pH梯度等电聚焦的影响 |
| |
| 引用本文： | 吴闯.缓冲液成分对固相pH梯度等电聚焦的影响[J].科学技术与工程,2012,12(33):9079-9081,9084. |
| |
| 作者姓名： | 吴闯 |
| |
| 作者单位： | 辽宁石油化工大学环境与生物工程学院,抚顺,113001 |
| |
| 摘 要： | 分别从水质、DTT(二硫苏糖醇)含量及含盐缓冲液三个方面对等电聚焦过程进行了对比。通过电学参数变化,研究了三者对聚焦进程的影响。结果表明:高质量的水、低含量的DTT和盐可以缩短聚焦时间,从而提高聚焦质量。可以把电学参数的变化作为优化和控制等电聚焦过程的指标,为正确设计合理的双向电泳实验流程提供依据。
|
| 关 键 词： | 等电聚焦 双向电泳 固相pH梯度 蛋白质组学 |
| 收稿时间： | 7/26/2012 9:41:36 PM |
| 修稿时间： | 8/14/2012 9:54:39 AM |
Effect of Buffer Composition on Isoelectric Focusing in Immobilized pH Gradients |
| |
| WU CHUANG.Effect of Buffer Composition on Isoelectric Focusing in Immobilized pH Gradients[J].Science Technology and Engineering,2012,12(33):9079-9081,9084. |
| |
| Authors: | WU CHUANG |
| |
| Institution: | WU Chuang(Liaoning Shihua University,Fushun 113001,P.R.China) |
| |
| Abstract: | Compared the process of IEF from three aspects of water quality, DTT dosage and salt content, and studied theit effects on isoelectric focusing by comparing the change of Electrical parameters. The results showed that high-quality water, low level of DTT and salt can shorten the focusing time, and then Improved the focus quality. Changes in the electrical parameters could be as the index of optimizing and controlling in isoelectric focusing. They provided the basis for designing perfect 2-DE protocol. |
| |
| Keywords: | isoelectric focusing 2-DE IPG proteomics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《科学技术与工程》浏览原始摘要信息 |
| 点击此处可从《科学技术与工程》下载免费的PDF全文 |
|
