栝楼毛状根生长及营养消耗动力学

为将毛状根用于根类中药材的工业化生产 ,研究了在不同接种量的条件下 ,栝楼 (Trichosanthes kirilowii)毛状根的生长及其对各种营养物质的消耗 ,同时考查了培养液的p H值和电导率的变化。实验结果表明 ,接种量不同 ,收获生物量基本相同 ,但毛状根的生长速度有差异 ,其生长倍数随着接种量的增大而降低。接种量越大毛状根对营养物质的消耗速度越快 ;毛状根的生长与碳源、氮和磷的消耗具有密切的相关性 ,毛状根快速生长期比营养物质快速消耗期滞后12 d左右。培养基的电导率变化与毛状根的生物量积累呈线性相关。

Kinetics of growth and nutrient consumption in a culture of Trichosanthes kirilowii hairy root

Ribosome inactiviting protein can be obtained by culturing Trichosanthes kirilowii hairy root. The growth and the nutrient consumption in the culture of Trichosanthes kirilowii hairy root were studied for various inoculum concentrations. The medium pH and conductivity were measured during the culture process. Though the inoculum concentrations differed, the harvested dry weight in the experiment groups had no obvious difference. However, the ratio of harvested dry weight to the initial dry weight decreased as the inoculum concentration increased. The results show that hairy roots with more inocula has higher nutrient consumption rate. The growth of hairy roots closely correlated with the consumption of sugar, nitrate, and phosphate. The log phase of the hairy root growth was about 12 d later than the high speed phase of nutrient consumption. The medium conductivity decreased linearly relative to the biomass accumulation.