| 马血清破伤风抗毒素分离纯化的研究 |
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| 引用本文： | 褚洪标,胡有生,彭才圣,梁兆昌,刘永青.马血清破伤风抗毒素分离纯化的研究[J].井冈山大学学报（自然科学版）,2011(4):98-100. |
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| 作者姓名： | 褚洪标 胡有生 彭才圣 梁兆昌 刘永青 |
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| 作者单位： | 井冈山大学医学院; |
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| 基金项目： | 吉安市科技局重点科技计划项目(吉市科计字[2009]40) |
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| 摘 要： | 目的提高马血清破伤风抗毒素制剂中有效成分的含量.方法在超滤浓缩之后,利用凝胶色谱对原液进行纯化,利用电泳图谱对结果进行分析.结果葡聚糖凝胶(Sephadex G-150)对抗毒素的分离有较好效果,可去除一部分杂蛋白,提高F(ab)2等有效成分的含量.结论利用凝胶色谱对抗毒素进行分离可提高制品质量,降低不良反应,因此增加...
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| 关 键 词： | 破伤风抗毒素 凝胶色谱 G-150 分离 纯化 |
SEPARATION AND PURIFICATION OF HORSE SERUM TETANUS ANTITOXINS |
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| CHU Hong-biao,HU You-sheng,PENG Cai-sheng,LIANG Zhao-chang,LIU Yong-qing.SEPARATION AND PURIFICATION OF HORSE SERUM TETANUS ANTITOXINS[J].Journal of Jinggangshan University(Natural Sciences Edition),2011(4):98-100. |
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| Authors: | CHU Hong-biao HU You-sheng PENG Cai-sheng LIANG Zhao-chang LIU Yong-qing |
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| Institution: | CHU Hong-biao,HU You-sheng,PENG Cai-sheng,LIANG Zhao-chang,LIU Yong-qing(School of Medicine,Jinggangshan University,Ji'an,Jiangxi 343000,China) |
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| Abstract: | Objective:To improve the content of active constituents in tetanus antitoxins.Methods:After ultrafiltration,the stock solution was purified by gel chromatography method and the results were analyzed by electrophoresis.Results:The contents of F(ab)2 were improved by use of Sephadex G-150 and the impurity proteins were decreased.Conclusion:The product quality was promoted by gel chromatography and the seperation technics may be feasible. |
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| Keywords: | tetanus antitoxins gel chromatography G-150 separation purification |
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