| 3种杜鹃属植物原生质体制备纯化条件的研究 |
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| 引用本文： | 涂艺声,熊立亭,冷冰. 3种杜鹃属植物原生质体制备纯化条件的研究[J]. 江西师范大学学报(自然科学版), 2009, 33(2) |
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| 作者姓名： | 涂艺声 熊立亭 冷冰 |
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| 作者单位： | 江西师范大学,生命科学学院,教育部鄱阳湖生态环境与资源研究重点实验室,江西,南昌,330022;江西师范大学,生命科学学院,教育部鄱阳湖生态环境与资源研究重点实验室,江西,南昌,330022;江西师范大学,生命科学学院,教育部鄱阳湖生态环境与资源研究重点实验室,江西,南昌,330022 |
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| 基金项目： | 江西省教育厅重点计划 |
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| 摘 要： | 以云锦杜鹃、西洋杜鹃、锦绣杜鹃的组培苗叶片为试验材料,研究了酶液组成、酶解时间、酶液pH值、材料生理状态对其原生质体分离效果的影响以及离心速度和离心时间对原生质体收集纯化的影响,实验结果表明:杜鹃组培苗叶原生质体分离最佳条件是酶液组成为2%纤维素酶+1%果胶酶,酶解总时间为14 h,酶液pH值为6.0;杜鹃原生质体收集纯化的离心适宜条件为1 000 r/min转速下离心6 min,能收集到大量有活力的原生质体,有活力的原生质体达79.27%.
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| 关 键 词： | 杜鹃 无菌苗叶 原生质体分离 |
The Studies on Technique Conditions of Protoplast Isolation and Purification of Three Species of Rhododendron |
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| TU Yi-sheng,XIONG Li-ting,LENG Bing. The Studies on Technique Conditions of Protoplast Isolation and Purification of Three Species of Rhododendron[J]. Journal of Jiangxi Normal University (Natural Sciences Edition), 2009, 33(2) |
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| Authors: | TU Yi-sheng XIONG Li-ting LENG Bing |
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| Affiliation: | College of Life Science;Jiangxi Normal University;Ministry of Education Key Lab of Poyang Lake Ecological Environment and Resource Development;Nanchang 330022;China |
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| Abstract: | Factors affecting protoplast Isolation and purification of Rhododendron cultivars three species of R.fortunei,R.hybridum and R.pulchrum in vitro,including different combinations of enzyme mixture,dissolved time,pH value of enzyme liquid,material physiological state,centrifugal speed and time,were studied.The yield and viability of protoplast was measured,through different combination of enzyme,different dissolved times,and when the separation at low-speed of 1 000 r/min.The results showed that the optimised... |
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| Keywords: | Rhododendron leaves of sterile shoot protoplast isolation |
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