Expression of Aspergillus niger 9891 Endoinulinase in Pichia pastoris |
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| Institution: | Laboratory of Gene Engineering, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;Department of Pharmaceutical Science, University of Toronto, Toronto, Ontario M5S 2S2 CANADA |
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| Abstract: | An endoinulinase gene from Aspergillus niger 9891 (CGMCC0991) has been expressed in Pichia pastoris GS115 using pPIC9 vector. The recombinant endoinulinase was highly expressed and the optimization of the expression in a 7 liter of fermentor has been investigated. In fermented broth, the concentration of protein secreted is 2.15 mg/ml. The activity of endoinulinase is 1501 U/ml with sucrose as substrate and 291 U/ml with inulin as substrate, 105 and 273 times higher than that from the original strain respectively. |
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| Keywords: | Aspergillus niger endoinulinase inulin oligo-fructose Pichia pastoris |
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