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Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers
作者姓名:XIEHua  GUANRongxia  CHANGRuzhen  QIULijuan
作者单位:InstituteofCropGermplasmResources,ChineseAcademyofAgriculturalSclences/KeyLaboratoryofCropGermplasm&Biotechnology,MinistryofAgriculture,Beijing100081,China
摘    要:There are abundant soybean germplasm in China. In order to assess genetic diversity of Chinese summer soybean germplasm, 158 Chinese summer soybean accessions from the primary core collection of G max were used to analyze genetic variation at 67 SSR loci. A total of 460 alleles were detected, in which 414 and 419 alleles occurred in the 80 Huanghuai and the 78 Southern summer accessions, respectively. The average number of alleles per locus was 6.9 for all the summer accessions, and 6.2 for both Huanghuai and Southern summer accessions. Marker diversity (D) per locus ranged from 0.414 to 0.905 with an average of 0.735 for all the summer accessions, from 0.387 to 0.886 with an average of 0.708 for the Huanghuai summer accessions, and from 0.189 to 0.884 with an average of 0.687 for the Southern summer accessions. The Huanghuai and Southern summer germplasm were different in the specific alleles, allelic-frequencies and pairwise genetic similarities. UPGMA cluster analysis based on the similarity data clearly separated the Huanghuai from Southern summer soybean accessions, suggesting that they were different gene pools. The results indicate that Chinese Huanghuai and Southern summer soybean germplasm can be used to enlarge genetic basis for developing elite summer soybean cultivars by exchanging their germplasm.

关 键 词:遗传多样性  大豆  胚质  农作物  NDA  核酸  基因
收稿时间:17 December 2004

Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers
XIEHua GUANRongxia CHANGRuzhen QIULijuan.Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers[J].Chinese Science Bulletin,2005,50(6):526-535.
Authors:Hua Xie  Rongxia Guan  Ruzhen Chang  Lijuan Qiu
Institution:(1) Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture, 100081 Beijing, China;(2) Beijing Agro-biotechnology Research Center, 100089 Beijing, China
Abstract:There are abundant soybean germplasm in China. In order to assess genetic diversity of Chinese sum- mer soybean germplasm, 158 Chinese summer soybean ac- cessions from the primary core collection of G. max were used to analyze genetic variation at 67 SSR loci. A total of 460 alleles were detected, in which 414 and 419 alleles oc- curred in the 80 Huanghuai and the 78 Southern summer accessions, respectively. The average number of alleles per locus was 6.9 for all the summer accessions, and 6.2 for both Huanghuai and Southern summer accessions. Marker diver- sity (D) per locus ranged from 0.414 to 0.905 with an average of 0.735 for all the summer accessions, from 0.387 to 0.886 with an average of 0.708 for the Huanghuai summer acces- sions, and from 0.189 to 0.884 with an average of 0.687 for the Southern summer accessions. The Huanghuai and Southern summer germplasm were different in the specific alleles, allelic-frequencies and pairwise genetic similarities. UPGMA cluster analysis based on the similarity data clearly separated the Huanghuai from Southern summer soybean accessions, suggesting that they were different gene pools. The results indicate that Chinese Huanghuai and Southern summer soybean germplasm can be used to enlarge genetic basis for developing elite summer soybean cultivars by ex- changing their germplasm.
Keywords:Chinese summer soybean (Glycine max (L  ) Merr)  SSR  markers  Genetic diversity    
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