| 猴头菌原生质体制备条件的研究 |
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| 引用本文： | 马向东,周伏忠.猴头菌原生质体制备条件的研究[J].河南科学,1995,13(1):65-69. |
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| 作者姓名： | 马向东 周伏忠 |
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| 作者单位： | 河南农业大学，河南省科学院生物研究所 |
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| 摘 要： | 比较了不同酶液、酸碱度，渗透压稳定剂，菌龄和菌丝培养基成分等因素对猴头菌菌丝释放原生质体作用的影响。结果表明：在土豆培养基（ＰＤＰ）上静止浅层培养９６小时的菌丝，用２％的溶壁酶液，以０．６ｍｏｌ／ＬＭｇＳＯ４为渗透压稳定剂，在ＰＨ５．５的条件下酶解，获得的原生质体数最大。
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| 关 键 词： | 猴头菌 原生质体 制备条件 食用菌 |
THE REASEARCH OF MYCELIAL PROTOPLASTSFROM HERICIUM ERINACEUS |
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| Ma Hiangdong.THE REASEARCH OF MYCELIAL PROTOPLASTSFROM HERICIUM ERINACEUS[J].Henan Science,1995,13(1):65-69. |
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| Authors: | Ma Hiangdong |
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| Abstract: | he effect of such factors as enzyme systems, osmotic stabilizers, different PH. medin andage on the formation of the mycelia protoplasts were studied. High yields of protoplasts fromHericium erinaceus were obtained by using 2% Lywallzyme, 0.6mol/ L MgSO_4, PH5.5 and96hr old mycelia cultured on PDP media. |
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| Keywords: | Hericium erinaceus Protoplast Condition of preparation |
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