| 小麦幼苗微管结合蛋白MAP65s体外对微管聚合影响的研究 |
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| 引用本文： | 马爱珍,韩榕.小麦幼苗微管结合蛋白MAP65s体外对微管聚合影响的研究[J].实验室科学,2013,16(1):30-33. |
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| 作者姓名： | 马爱珍 韩榕 |
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| 作者单位： | 山西师范大学生命科学学院,山西临汾,041004 |
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| 基金项目： | 国家自然科学基金(项目编号:30671061);山西省自然科学基金(项目编号:2008011059-1) |
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| 摘 要： | 微管结合蛋白与微管特异地结合在一起,参与调节微管的结构与功能,目前已成为植物细胞骨架研究的前沿领域。以"临优2018"小麦幼苗为材料,通过免疫印迹实验鉴定了小麦幼苗中微管结合蛋白MAP65s的存在并利用紫外吸收法和SDS-聚丙烯酰胺凝胶电泳法初步探究了小麦幼苗MAP65s体外对微管聚合的影响,为进一步研究MAP65s蛋白的生理功能提供了重要的依据。
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| 关 键 词： | 小麦 微管 微管结合蛋白MAP65s |
Studies of microtubules polymerization in vitro affected by microtubule-associated protein MAP65s isolated from wheat seedlings |
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| MA Ai-zhen
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HAN Rong.Studies of microtubules polymerization in vitro affected by microtubule-associated protein MAP65s isolated from wheat seedlings[J].Laboratory Science,2013,16(1):30-33. |
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| Authors: | MA Ai-zhen HAN Rong |
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| Institution: | (College of Life Science,Shanxi Normal University,Linfen 041004,China) |
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| Abstract: | Microtubule-associated proteins with microtubules＇ specifically together, involved in the regulation of microtubules structure and function, have become the cutting-edge research of the plant cytoskeleton. In this study, the wheat seedlings of lin you 2018 were chosen as experimental material. MAP65s were extracted and researched by Western-Blot in order to identify their presence. The func- tions of MAP65s were studied by the method of Spectrophotometery and SDS-PAGE. It provides im- portant evidences for further functional studies of the MAP65s. Western- Blot assay showed that MAP65s existed in the wheat seedlings. |
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| Keywords: | wheat microtubules microtubule-associated protein MAP65s |
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