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2,4-D和6-BA对唐古特大黄愈伤组织诱导的影响
引用本文:张洋,陈志,王慧春.2,4-D和6-BA对唐古特大黄愈伤组织诱导的影响[J].北京教育学院学报(自然科学版),2006(2).
作者姓名:张洋  陈志  王慧春
作者单位:青海师范大学生命与地理科学学院 西宁810008
摘    要:实验选用唐古特大黄(Rheum tanguticum Maxim.ex Regel.)种子萌发的无菌苗为外植体进行离体培养。结果表明,2mg/L的2,4-D最有利于愈伤组织的形成,但更高浓度的2,4-D会影响诱导效果;1mg/L的6-BA有利于愈伤组织的快速诱导,但单独的6-BA无诱导作用。

关 键 词:唐古特大黄  愈伤组织  植物激素

Effect of 2,4-D and 6-BA on Callus Induction from Rheum Tanguticum
Zhang Yang Chen Zhi Wang Huichun.Effect of 2,4-D and 6-BA on Callus Induction from Rheum Tanguticum[J].Journal of Beijing Institute of Education,2006(2).
Authors:Zhang Yang Chen Zhi Wang Huichun
Abstract:Seed germinated seedlings of Rheum tanguticum Maxim.ex Regel.were used as explants cultured in vitro to induce the callus.The experiment results showed that 2mg/L 2,4-D was beneficial to the formation of callus,but higher concentration of 2,4-D had bad effect on callus induction.1mg/L 6-BA could accelerate the growth of the callus in the medium with 2,4-D,while 6-BA couldn't induce callus itself.
Keywords:Rheum tanguticum Maxim  ex Regel    callus  plant hormone
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