| 红豆杉单细胞平板培养的研究 |
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| 引用本文： | 李景原 韩书亮. 红豆杉单细胞平板培养的研究[J]. 河南师范大学学报(自然科学版), 1997, 25(1): 107-109 |
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| 作者姓名： | 李景原 韩书亮 |
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| 作者单位： | 河南师范大学生物系 |
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| 基金项目： | 河南省教委自然科学基金 |
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| 摘 要： | 本文报道了红豆杉单细胞平板培养的过程．研究了细胞悬浮培养时间、培养方式和接种密度对红豆杉单细胞平板培养植板率的影响．结果表明，以悬浮培养１０－１４ｄ的单细胞为材料，接种密度为３－５×１０３／ｍｌ时，进行条件培养和看护培养均能获得较高的植板率
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| 关 键 词： | 红豆杉；平板培养；植板率 |
Study on the Monocell Plate Culture of Taxus Chinensis |
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| Li Jingyuan Han Shuliang Li Faqi Wang Taixia. Study on the Monocell Plate Culture of Taxus Chinensis[J]. Journal of Henan Normal University(Natural Science), 1997, 25(1): 107-109 |
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| Authors: | Li Jingyuan Han Shuliang Li Faqi Wang Taixia |
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| Abstract: | Factors such as cell suspension culture time,the ways of plating culture and cell density,which affects the plating efficiency were investigated in this paper.The result showed that plating at density of 3-5×10 3cells/ml and with monocells seperated from 10-14 day cell,high plating efficiency could be botained either by conditional plate culture or by nursing plate culture. |
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| Keywords: | Taxus Chinensis Plate culture plating efficiency |
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