氯霉素分子烙印固相萃取柱的制备及萃取条件优化

采用分子烙印技术制备了氯霉素的分子烙印固相萃取柱，并通过对其吸附能力、解吸条件等特性的研究，确定了分子烙印固相萃取柱的上柱、淋洗和洗脱条件．结果表明，与目前氯霉素检测中所采用的传统C18柱相比，分子烙印固相萃取柱可采用甲醇含量较高的甲醇／水混合溶剂作为淋洗溶剂，用乙腈含量为40％(体积分数)的乙腈／水作为洗脱溶剂，且比传统C18柱具有更大的柱容量．分子烙印固相萃取柱的优点使其可应用于实际生物样品中氯霉素的检测，以解决目前氯霉素残留检测中面临的难题．

Preparation of Chloramphenicol Molecularly Imprinted Solid-phase Extraction Cartridge and the Optimization of Extraction Conditions

The molecularly imprinted solid-phase extraction (SPE) cartridge of chloramphenicol(CAP) was prepared by adopting molecular imprinting technique,and its absorption ability as well as desorption conditions was investigated,on the basis of which the working conditions of SPE cartridge,including the solvent for CAP,the rinse and elution conditions,were ascertained.The results indicate that,compared to the traditional C18 cartridges,the molecularly imprinted solid-phase extraction cartidge is of great advantages,that is,it can use methanol-water solution with high methanol content as the rinse solution,acetonitrile-water solution with 40%(volume fraction) acetonitrile as the elution solution,and it is of a greater cartridge capacity than C18 cartridges.All these advantages make it possible to apply the SPE cartridge to the CAP detection of actual bio-products,thus resolving the problems existing in the CAP residue detection at present.