| 红细胞表面电荷的研究 Ⅲ.红细胞年龄对电泳率的影响 |
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| 引用本文： | 秦德安,何学民,钮晓达. 红细胞表面电荷的研究 Ⅲ.红细胞年龄对电泳率的影响[J]. 华东师范大学学报(自然科学版), 1985, 0(3) |
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| 作者姓名： | 秦德安 何学民 钮晓达 |
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| 作者单位： | 华东师范大学生物系(秦德安，何学民)，华东师范大学生物系(钮晓达) |
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| 摘 要： | 本文采用Murphy法分离老、中、青不同年龄的红细胞.经细胞电泳测定,人红细胞电泳率随细胞年龄增大而下降(P<0.01).并进一步证实,人红细胞在体内衰老过程中,其电泳率的下降和唾液酸含量减少有关.
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| 关 键 词： | 红细胞衰老 红细胞电泳率 细胞电泳。 |
Studies on Erythrocyte Surface Charges III. Influence of Different Aged Human Erythrocytes on Their Electrophoretic Mobility |
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| QIN DEAN, HE XUEMIN, NIU XIAODA. Studies on Erythrocyte Surface Charges III. Influence of Different Aged Human Erythrocytes on Their Electrophoretic Mobility[J]. Journal of East China Normal University(Natural Science), 1985, 0(3) |
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| Authors: | QIN DEAN HE XUEMIN NIU XIAODA |
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| Affiliation: | Department of Biology |
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| Abstract: | Different aged human erythrocytes were separated with Murphy's method (1973).After centrifuging, the red cell column was divided into ten fractions which were numbered from the top.Thus, fraction 1 contained the youngest population of cells,fraction 6 the middle, and fraction 10 the most aged ones. Erythrocyte mobility of different ages were measured electrophoreticly in a square glass capillary chamber.Results suggested the electrophoretic mobility and the sialic acid content of human erythrocyte were decreased with ageing (P<0.01). It is again assumed that during the process of ageing, the electrophoretic mobility reduction of human erythrocyte is related to the loss of sialic acid which presents on its surface. |
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| Keywords: | erythrocyte ageing erythrocyte mobility cell electrophoresis. |
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