| 等边浅蛤(Gomphina aequilatera)核糖体DNA第一内转录间隔区序列的特征分析 |
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| 引用本文： | 吕锋骅,韩洁,林岿璇,陈万东.等边浅蛤(Gomphina aequilatera)核糖体DNA第一内转录间隔区序列的特征分析[J].北京师范大学学报(自然科学版),2011(4):398-404. |
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| 作者姓名： | 吕锋骅 韩洁 林岿璇 陈万东 |
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| 作者单位： | 生物多样性与生态工程教育部重点实验室北京师范大学生命科学学院;南麂列岛国家级海洋自然保护区; |
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| 基金项目： | 北京师范大学优秀博士学位论文培育基金资助项目(10420801); 浙江省温州市科技局研究资助项目(S2005A004) |
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| 摘 要： | 从浙江和福建沿海潮间带的6个采样点各随机选取2个等边浅蛤(Gomphina aequilatera)样品,以聚合酶链式反应、分子克隆和序列测定等分子生物学实验技术获得了ITS-1序列.通过比对发现,该序列内存在一个长度为17 bp的插入/缺失片段和一个结构为(GA)3(GGGA)2(GA)4~6的微卫星DNA,这2个结构是造成等边浅蛤ITS-1序列在个体基因组内及个体之间长度变化的主要原因.统计分析结果显示,该序列在个体基因组内的变异与相同采样点个体之间的变异无显著差异(P>0.05),而与不同采样点个体之间的变异差异显著(P<0.05),因此在种群水平的研究中是有效的分子标记.系统发生关系重建的结果和单倍型网络结构图均显示等边浅蛤的ITS-1序列各单倍型间的亲缘关系较近.
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| 关 键 词： | ITS-1 分子标记 序列变异 等边浅蛤 |
CHARACTERIZATION OF INTERNAL TRANSCRIBED SPACER ONE IN GOMPHINA AEQUILATERA RIBOSOMAL DNA |
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| Lü Fenghua,HAN Jie,LIN Kuixuan,CHEN Wandong.CHARACTERIZATION OF INTERNAL TRANSCRIBED SPACER ONE IN GOMPHINA AEQUILATERA RIBOSOMAL DNA[J].Journal of Beijing Normal University(Natural Science),2011(4):398-404. |
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| Authors: | Lü Fenghua HAN Jie LIN Kuixuan CHEN Wandong |
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| Institution: | Lü Fenghua1) HAN Jie1) LIN Kuixuan1) CHEN Wandong2)(1) Key Laboratory for Biodiversity Science and Ecological Engineering of Ministry of Education,College of Life Sciences: Beijing Normal University,100875,Beijing,China,2) Nanji Islands National Marine Natural Reserve Administration,325014,Wenzhou,Zhejiang,China) |
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| Abstract: | Internal transcribed spacer one(ITS-1) in ribosomal DNA is a of the molecular marker frequently used in phylogeny,population genetics and species taxonomy studies.However,the presence of variations among multiple copies within individual genome is often neglected.In this study,Gomphina aequilatera ITS-1 sequences of two individual subjects from six sampling sites each at intertidal zone along Zhejiang and Fujian provinces were obtained by polymerase chain reaction,molecular cloning and sequencing.Both an 17... |
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| Keywords: | ITS-1 molecular marker sequence variation Gomphina aequilatera |
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