| 暗纹东方鲀（Takifugu obscurus）性别分化的组织学及芳香化酶基因表达研究 |
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| 引用本文： | 李延伸,戴奇,郭正龙,朱永祥,黄波,周忠良. 暗纹东方鲀（Takifugu obscurus）性别分化的组织学及芳香化酶基因表达研究[J]. 复旦学报(自然科学版), 2011, 0(5): 645-652 |
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| 作者姓名： | 李延伸 戴奇 郭正龙 朱永祥 黄波 周忠良 |
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| 作者单位： | 华东师范大学生命科学学院;江苏中润农业发展有限公司; |
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| 摘 要： | 采用组织切片技术和实时定量PCR（qRT-PCR）方法研究了暗纹东方鲀性别分化过程中性腺发育的组织学及芳香化酶基因CYP19A的表达变化.结果显示：孵化后11d的仔鱼切片中可看到原始生殖细胞（PGC）;孵化后17d可看到隆起的性腺原基（GA）;孵化后26d发育成为原始性腺（PG）并从体腔膜上游离出来.卵巢分化时间早于精...
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| 关 键 词： | 暗纹东方鲀 性别分化 组织学分析 CYP19A |
Histological Analysis and Aromatase Gene Expression in Sex Differentiation in Takifugu obscures |
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| LI Yan-shen,DAI Qi,GUO Zheng-long,ZHU Yong-xiang,HUANG Bo,ZHOU Zhong-liang. Histological Analysis and Aromatase Gene Expression in Sex Differentiation in Takifugu obscures[J]. Journal of Fudan University(Natural Science), 2011, 0(5): 645-652 |
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| Authors: | LI Yan-shen DAI Qi GUO Zheng-long ZHU Yong-xiang HUANG Bo ZHOU Zhong-liang |
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| Affiliation: | LI Yan-shen1,DAI Qi1,GUO Zheng-long2,ZHU Yong-xiang2,HUANG Bo1,ZHOU Zhong-liang1(1.School of Life Science,East China Normal University,Shanghai 200062,China,2.Jang Su Zhong Run Agricultural Development Co.,Ltd.,Nantong 226634,China) |
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| Abstract: | The process of gonadal differentiation and Aromatase gene CYP19A expression in Takifugu obscures was studied by histological analysis and quantitative real-time PCR(qRT-PCR).The results of histological analysis showed: primordial germ cells(PGC) was observed at 11 dah(day after hatching),and gonad anlage(GA) occurred at 17 dah;Primitive gonad(PG) freed from the coelarium was observed at 26 dah.Ovarian differentiation occurred earlier than testicular.The original ovarian cavity(Oc) appeared in some individua... |
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| Keywords: | Takifugu obscures sex differentiation histological analysis CYP19A |
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