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Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy |
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| Authors: | Mi Li LianQing Liu Ning Xi YueChao Wang ZaiLi Dong GuangYong Li XiuBin Xiao WeiJing Zhang |
| Affiliation: | LI Mi~1,2,LIU LianQlng~1*,XI Ning~1,3*,WANG YueChao~1,DONG ZaiLi~1,LI GuangYong~1,4,XIAO XiuBin~5 & ZHANG WeiJing~5.1 State Key Laboratory of Robotics,Shenyang Institute of Automation,Chinese Academy of Sciences,Shenyang 110016,China,2 Graduate University of Chinese Academy of Sciences,Beijing 100049,3 Department of Electrical and Computer Engineering,Michigan State University,East Lansing 48824,USA,4 Department of Electrical and Computer Engineering,University of Pittsburgh,Pittsburgh 15261,Depar... |
| Abstract: | The invention of atomic force microscopy(AFM) has provided new technology for measuring specific molecular interaction forces.Using AFM single-molecule force spectroscopy(SMFS) techniques,CD20-Rimximab rupture forces were measured on purified CD20 proteins,Raji cells,and lymphoma patient B cells.Rimximab molecules were linked onto AFM tips using AFM probe functionalization technology,and purified CD20 proteins were attached to mica using substrate functionalization technology.Raji cells(a lymphoma cell line... |
| Keywords: | atomic force microscopy single-molecule force spectroscopy non-Hodgkin's lymphoma monoclonal antibody |
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