白花菜子不同溶剂提取物总多酚、总黄酮含量及抗氧化活性测定

测定了白花菜子四种不同溶剂提取物的总多酚含量、总黄酮含量和体外抗氧化活性。以没食子酸为对照品,采用福林-酚试剂法测定白花菜子提取物中总多酚的含量;以芦丁为对照品,采用可见-紫外分光光度法测定白花菜子提取物中总黄酮的含量。通过测定DPPH自由基清除率、氢氧基清除率和还原能力来评价白花菜子提取物的抗氧化活性。在四种白花菜子不同溶剂提取物中,总黄酮含量从高到低的顺序为乙酸乙酯提取物(CAEE)甲醇提取物(CME)石油醚提取物(CPEE)二氯甲烷提取物(CCE);总多酚含量从高到低的顺序为CMECAEECPEECCE。白花菜子提取物各种方法抗氧化活性从高到低的顺序为CMECAEECPEECCE,与总多酚含量顺序一致。结果表明白花菜子乙酸乙酯提取物总黄酮含量最高,白花菜子甲醇提取物总多酚含量最高,且甲醇提取物具有很强的抗氧化活性,可以用作抗氧化药物或食品添加剂,因此有必要对白花菜子的化学成分、体内抗氧化活性和机理进行深一步的研究。

Determination of Total Phenolics Contents, Total Flavonoids Contents and Antioxidant Activities of Four Different Solvent Extracts of Cleome gynandra L. seeds

In this study, total phenolics contents, total flavonoids contents and antioxidant activities of four different solvent extracts of Cleome gynandra L. seeds were determined. Total phenolics contents of the extracts prepared were determined with Folin-Ciocalteau reagent according to the method using gallic acid as a standard phenolic compound. The total flavonoids contents of the extracts prepared were determined by UV spectrophotometry and rutin was used to make the calibration curve. Antioxidant activities of the extracts prepared were evaluated applying DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, total reducing power and hydroxyl ion scavenging assay. The decreasing order of total flavonoids contents is acetate ethyl extract (CAEE) > methanol extract (CME) > petroleum ether extract (CPEE) > dichloromethane extract (CCE) in four different solvent extracts. The decreasing order of total phenolics contents is CME > CAEE > CPEE > CCE. The decreasing order of antioxidant activities is CME > CAEE > CPEE > CCE in all the methods which is in conformity with total phenolic content. The results clearly demonstrate that acetate ethyl extract has highest total flavonoids content, but methanol extract has highest total phenolics content and strongest antioxidant activities. It can be used to prevent oxidative stress related diseases and can be stored as food supplement. So further investigation of individual isolated compounds and their antioxidant activities in vivo and different antioxidant mechanisms is needed.