Studies inin situ PCR detected by the BrdU antibody technique |
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Authors: | Zhang Xiyuan Jiang Haibo Ma Qi Yang Jianqi Li Lijia Liu Ting Yan Ju |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China;(2) Genetics Lab of Tongji Medical University, 430030 Wuhan |
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Abstract: | Using the BrdU antibody technique followed by an immuno-chemical staining (BAT), the amplification of DNA fragments specific
to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products
orin situ hybridization with PCR products on slides, the amplified target DNA fragments of specimen were visualized by BAT under the
microscope. The availability of BAT and differences in the sensitivity and efficiency between BAT and dig-11-dUTP labeling
in cellin situ PCR were discussed.
Zhang Xiyuan: born in Oct. 1935, Professor, Current research interest in Cellular and Molecular Biology
Supported by the National Natural Science Foundation of China |
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Keywords: | BrdU antibody technique cellin situ PCR in situ hybridization human Y chromosome polymerase chain reaction (PCR) |
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