| IGF-Ⅱ在原核生物中的表达 |
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| 引用本文： | 王庆忠,任科云. IGF-Ⅱ在原核生物中的表达[J]. 潍坊学院学报, 2010, 10(6): 103-105,160 |
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| 作者姓名： | 王庆忠 任科云 |
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| 作者单位： | 潍坊学院,山东潍坊261061 |
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| 摘 要： | 胰岛素生长因子IGF-Ⅰ和IGF-Ⅱ在机体的许多组织中都有表达,具有促进细胞生长和分裂等的多种生物学功能。在本研究中,我们采用RT-PCR法克隆和扩增到了IGF-Ⅱ基因,通过双酶切、胶回收纯化、连接后得到了pET21b-IGF-Ⅱ重组质粒载体,然后将其转化DH5α中,经IPTG诱导后表达的重组蛋白经纯化,并用Western blot检测,显示重组质粒pET21b-IGF-Ⅱ编码一个约25kD的外源蛋白。
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| 关 键 词： | IGF-Ⅱ RT-PCR 原核表达载体 表达 |
IGF-II Expressed in Prokaryotic Organism |
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| WANG Qing-zhong,REN Ke-yun. IGF-II Expressed in Prokaryotic Organism[J]. Journal of Weifang University, 2010, 10(6): 103-105,160 |
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| Authors: | WANG Qing-zhong REN Ke-yun |
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| Affiliation: | (Weifang University,Werfang 261061,China) |
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| Abstract: | IGF-Ⅰand IGF-Ⅱ are expressed in many organs,and have important roles in cell growth and proliferation.In this study,we,the target gene,IGF-Ⅱ,was cloned and amplified By RT-PCR method from cardiac muscle and then inserted into T-vector,and then inserted the expression vecter pET21b.The recombinant vector was indicated expressing IGF-Ⅱprotein in E.coli DH5α after induced by IPTG.We then purified reeombinant protein and analyzed via Western blot. |
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| Keywords: | IGF-Ⅱ RT-PCR prokaryotic expression vector expression |
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