Intrinsic fluorescence of mitochondrial F1-ATPase

Summary The emission maximum of the fluorescence spectrum of the mitochondrial F₁-ATPase in shifted from 305 to 334 nm when the excitation wavelength is altered from 270 to 300 nm. This indicates that both tyrosine and tryptophan contribute to the intrinsic fluorescence of the F₁-ATPase.Acknowledgments. The authors wish to thank F. Penin for his preparation of F₁-ATPase and Drs J. M. Jallon and N. Gains for their stimulating discussions. This research was supported by the CNRS and by the DGRST (contract 77-7-0277).