酶法结合膜技术制备高效价肝素钠的工艺研究

以肝素钠的效价为指标,以猪小肠黏膜为原料,从中提取抗凝血药物肝素钠,提出酶法结合膜技术提取肝素钠;并将纯化工程中的传统静态树脂吸附改为动态吸附,与传统工艺相比。在此工艺条件下,酶解后产品的效价提高了33%,最后收率可提高了34.7%。加入膜技术除去杂蛋白和核酸后,以效价衡量产品纯度,对比粗品提高了125%,对比精品提高了20%。

Research on High Efficiency Price Heparin Sodium with Enzymatic Combined Membrane Preparation Technology

In this paper titer as index to the heparin sodium, small intestinal mucosa with pig as raw materials, to extract the anti-clotting drug heparin sodium, put forward a kind of innovation new technology with enzymatic combined membrane to extract heparin sodium, and changed the traditional static adsorption to dynamic adsorption in the purification engineering of resin. Compared the traditional process of the results: enzyme solution of the products after effect price increased by 33%, the yield of the last increased 34.7%, add membrane technology to remove noise protein and nucleic acid in titer measure, With valence measure product purity, contrasted crude products increased by 125%, compared to high-quality goods increased by 20%.