| 鹿鞭及其伪品随机扩增DNA多态性鉴定方法研究 |
| |
| 引用本文： | 盛瑜,孙景昱,李明成,张丽华,李洪宇,李梓僮,苑广信.鹿鞭及其伪品随机扩增DNA多态性鉴定方法研究[J].北华大学学报(自然科学版),2015,16(5):605-608. |
| |
| 作者姓名： | 盛瑜 孙景昱 李明成 张丽华 李洪宇 李梓僮 苑广信 |
| |
| 作者单位： | 北华大学药学院,吉林 吉林,132013;吉林市中心医院,吉林 吉林,132001;北华大学医学检验学院,吉林 吉林,132013 |
| |
| 基金项目： | 吉林省教育厅科学技术研究项目(2012143;2014212)，吉林省战略性新兴产业和高新技术产业发展专项基金(2013G030 |
| |
| 摘 要： | 目的建立鹿鞭及其伪品随机扩增DNA多态性(RAPD)鉴定方法,为鹿鞭的鉴别提供可靠依据.方法采用柱层析法从鹿鞭及其伪品中提取线粒体DNA并进行随机DNA多态性扩增.结果正品鹿鞭(梅花鹿鞭和马鹿鞭)与其常见伪品(牛鞭)线粒体DNA随机扩增产物的电泳图谱有明显区别,条带数量和位置均不同,说明本方法可以有效区分正品鹿鞭与其常见伪品.结论 RAPD技术可为鹿鞭及其伪品的鉴别提供一种简单、有效、可信度高的方法.
|
| 关 键 词： | 梅花鹿鞭 马鹿鞭 牛鞭 RAPD 指纹图谱 |
On Application of RAPD Technology on Penis Cervi Identification Based on mt DNA |
| |
| Abstract: | Objective To establish the identification method of Penis Cervi from its adulterants by RAPD technology,and to provide a reliable evidence for Penis Cervi identification. Method Column chromatography method was used to extract and purify mt DNAs from Penis Cervi and its adulterants,and the DNA fragments were amplified by RAPD technology. Results The results showed that there was a great difference in the number and brightness of the stripes of RAPD products between Penis Cervi ( Gervus nippon Temminck whip and Cervus elaphus Linnaeus whip) and its adulterants(Bull whip). Conclusion The technology of RAPD could be a simple,effective and reliable method for the identification of Penis Cervi from its adulterants. |
| |
| Keywords: | Gervus nippon Temminck whip cervus elaphus Linnaeus whip bull whip RAPD fingerprint |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
