| 纤维素酶在葛根提取工艺中的应用 |
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| 引用本文： | 杨云,万焱,张寒娟,贺海花.纤维素酶在葛根提取工艺中的应用[J].河南科学,2008,26(12). |
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| 作者姓名： | 杨云 万焱 张寒娟 贺海花 |
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| 作者单位： | 河南中医学院,药学院,郑州,450008 |
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| 基金项目： | 国家"十一五"科技支撑计划 |
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| 摘 要： | 为研究纤维素酶对葛根提取工艺的影响,采用平行对比实验和正交设计,并以薄层扫描法对葛根提取物进行含量测定.结果显示,用纤维素酶解后,葛根素含量提高175.9%,影响酶解的因素为温度、时间、pH值、用酶量.
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| 关 键 词： | 纤维素酶 正交试验 提取 含量测定 葛根素 |
Research on Application of Cellulose Enzyme in the Extraction of Pueraria |
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| Yang Yun,Wan Yan,Zhang Hanjuan,He Haihua.Research on Application of Cellulose Enzyme in the Extraction of Pueraria[J].Henan Science,2008,26(12). |
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| Authors: | Yang Yun Wan Yan Zhang Hanjuan He Haihua |
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| Institution: | Yang Yun,Wan Yan,Zhang Hanjuan,He Haihua(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China) |
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| Abstract: | To research the effect of cellulose enzyme on the extract technics of pueraria. We adopt parallel experiments and orthogonal design,use the method of TLCS to determine the contents of the extracted material in pueraria. After the use of cellulose enzyme,the content of puerarin in the extracted material had increased by 175.9 %. The facts effecting the enzyme-hydrolyze are temperature,time,pH,the cost of enzyme. |
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| Keywords: | cellulose enzyme orthogonal design extract content determine puerarin |
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