| 钙离子敏感受体基因cDNA的克隆与序列分析 |
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| 引用本文： | 郭淑华. 钙离子敏感受体基因cDNA的克隆与序列分析[J]. 潍坊学院学报, 2005, 5(4): 92-94 |
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| 作者姓名： | 郭淑华 |
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| 作者单位： | 山东师范大学,山东,济南,250014 |
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| 摘 要： | 本研究通过RT-PCR技术从妊娠第九天的大鼠子宫中扩增出长为423bp的钙离子敏感受体cDNA片段,PC R产物经凝胶回收纯化后,克隆在PGEM-T载体的T位点.测序结果和序列分析表明,本试验已成功地扩增、克隆了钙离子敏感受体基因cDNA序列,为进一步研究钙离子敏感受体在大鼠子宫中的表达与调节奠定了基础.
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| 关 键 词： | 钙离子敏感受体 克隆 |
| 文章编号： | 1671-4288（2005）04-0092-03 |
| 收稿时间： | 2004-12-16 |
| 修稿时间： | 2004-12-16 |
Cloning and sequence analysis of Calcium-sensing receptorgene Cdna |
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| GUO Shu-hua. Cloning and sequence analysis of Calcium-sensing receptorgene Cdna[J]. Journal of Weifang University, 2005, 5(4): 92-94 |
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| Authors: | GUO Shu-hua |
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| Abstract: | By RT-PCR technology,423bpCaR gene cDNA was amplified from Pregnancy Rat Uterus on nine day and cloned in T site of PGEM-T Vector.The sequencing results and sequence analysis showed that Calcium-sensing receptor gene cDNA sequence was succeed in amplifying and cloning. |
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| Keywords: | RT-PCR |
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