| 麻疯树毒蛋白(curcin)基因转化水稻的研究 |
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| 引用本文： | 廖毅,魏琴,徐莺,王胜华,唐琳,白洁,陈兴春,陈放. 麻疯树毒蛋白(curcin)基因转化水稻的研究[J]. 四川大学学报(自然科学版), 2006, 43(6): 1399-1404 |
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| 作者姓名： | 廖毅 魏琴 徐莺 王胜华 唐琳 白洁 陈兴春 陈放 |
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| 作者单位： | 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 国际合作项止“中国西南地区能源植物的开发和利用”(No.2004DFB00300) |
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| 摘 要： | 以粳稻(Japonica)品种日本晴与中花11号为材料,对影响农杆菌介导水稻转化体系的几个因素进行了研究,建立了农杆菌介导的有效水稻转化体系.在该体系中,首次使用浓度为10-4g/mL嘌呤合成抑制剂acivicin在转化前对水稻愈伤组织进行预处理,结果表明此法可有效提高GUS基因的瞬时表达率.同时,利用插入了麻疯树毒蛋白(curcin)基因的植物表达质粒pBI121-curcin,通过农杆菌介导转化水稻,获得了多株转基因植株.通过PCR鉴定,证实curcin基因已整合到水稻基因组中.
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| 关 键 词： | 水稻 农杆菌 转化 acivicin 麻疯树毒蛋白基因 |
| 文章编号： | 0490-6756（2006）06-1399-06 |
| 收稿时间： | 2006-04-03 |
| 修稿时间： | 2006-04-03 |
Study on Transformation of Rice for Curcin Gene of Jatropha curcas |
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| LIAO Yi,WEI Qin,XU Ying,WANG Sheng-hua,TANG Lin,BAI Jie,CHEN Xing-chun,CHEN Fang. Study on Transformation of Rice for Curcin Gene of Jatropha curcas[J]. Journal of Sichuan University (Natural Science Edition), 2006, 43(6): 1399-1404 |
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| Authors: | LIAO Yi WEI Qin XU Ying WANG Sheng-hua TANG Lin BAI Jie CHEN Xing-chun CHEN Fang |
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| Affiliation: | College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University,College of Life Sciences; Sichuan University |
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| Abstract: | Several important factors have been investigated on affecting Agrobacterium-mediated rice transformation system by using rice cultivars Nipponbare and Zhonghua No.11,and an efficient Agrobacterium-mediated rice transformation system was developed.In this system,it was found that the GUS transient expression of transformed rice calli was improved by prior exposure to 10~(-4)g/mL acivicin,which is a kind of purine synthesis inhibitors,before infection with A.tumefaciens.At the same time,using the plasmid pBI1 |
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| Keywords: | Oryza sativa L. Agrobacterium tumefaciens transformation acivicin curcin gene |
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