| 用分子探针探测两种AM真菌在同一宿主植物根内的侵染 |
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| 引用本文： | 董昌金.用分子探针探测两种AM真菌在同一宿主植物根内的侵染[J].湖北师范学院学报(自然科学版),2004,24(2):4-6. |
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| 作者姓名： | 董昌金 |
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| 作者单位： | 湖北师范学院,生物系,湖北,黄石,435002 |
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| 摘 要： | 两种AM菌根真菌(G．intraradices和G．mosseae)混合接种于同一宿主植物紫云英中，通过Trypan blue染色检测了AM真菌在紫云英根中的存在，并通过nested PCR和特异性的分子探针，探测了Gintraradices和G．mosseae对紫云英同一根段的侵染。
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| 关 键 词： | 分子探针 AM真菌 宿主植物 侵染 Trypan blue染色 nested PCR 丛枝菌根 |
| 文章编号： | 1009-2714(2004)02-0004-03 |
| 修稿时间： | 2003年12月29 |
Infection of two fungi species for the host roots was probed by molecular probes |
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| DONG Chang,jin.Infection of two fungi species for the host roots was probed by molecular probes[J].Journal of Hubei Normal University(Natural Science),2004,24(2):4-6. |
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| Authors: | DONG Chang jin |
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| Abstract: | In this study,the host plant Astragalus sinicus was inoculated by two AM fungi species(G.intraradices and G.mosseae).The results of Trypan blue staining showed that AM fungi infected the roots of Astragalus sinicus.G.intraradices and G.mosseae which consisted in the same root of Astragalus sinicus were probed by the nested PCR and specific molecular probes. |
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| Keywords: | AM fungi infection trypan blue staining molecular probe nested PCR |
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