氯苯高效降解菌固定化小球的制备及其降解条件研究

以从大连瑞泽农药公司的活性污泥中新分离到的1株氯苯高效降解菌作为研究对象,以海藻酸钠（SA）为包埋剂,以氯化钙（CaCl2）为固定剂制成固定化小球.通过研究不同包埋条件对固定化小球性能的影响,确定了最佳固定化参数：海藻酸钠溶液的质量浓度为4%,氯化钙溶液的质量浓度为3%,交联时间为10 min.此外还研究了固定化小球在不同氯苯浓度、温度、pH和摇床转速下对氯苯降解的影响.结果表明,当氯苯浓度为120 mg/L、温度为30℃、pH为7.0、摇床转速为120 r/min时,固定化小球对氯苯的降解效果最好.

Preparation of immobilized microorganisms degrading chlorobenzene and reaserch of degrading conditions

A new strain of high-efficiency degrading chlorobenzene was isolated from the activated sludge of Dalian Ruize pesticide company, using sodium-alginate （SA） as embedding material and calcium chloride as immobilizing material. The optimal parameters were determined by studying the in- fluence of different embedding conditions on the performance of immobilized cells： SA 4%, CaCl2 3% and the cross-linked time 10 min. The influence of the immobilized cells on chlorobenzene degrading ability of the immobilized cell were studied, in various conditions such as chlorobenzene mass concertration, cultivatable temperature, pH and shaking table revolution. The results show that the optimal degradation conditions were as follows： the concentration of chlorobenzene is 120 mg/L, temperature is 30 ℃, pH is 7.0, rotating rate is 120 r/min.