| 不同功能化量子点与细胞的非特异性作用研究 |
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| 引用本文： | 王 青,张鹏飞,羊小海,王柯敏,武春雷.不同功能化量子点与细胞的非特异性作用研究[J].湖南大学学报(自然科学版),2010,37(5):60-63. |
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| 作者姓名： | 王 青 张鹏飞 羊小海 王柯敏 武春雷 |
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| 作者单位： | 湖南大学,化学生物传感与计量学国家重点实验室,化学化工学院,生物纳米与分子工程湖南省重点实验室,湖南,长沙,410082 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，湖南省高等学校青年骨干教师培养基金，湖南省杰出青年科学基金 |
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| 摘 要： | 选择COS-7细胞为研究对象,考察了不同功能化基团修饰的量子点(QDs)与细胞的非特异性相互作用.结果表明,氨基聚乙二醇衍生磷脂修饰的量子点(NH2-PEG-QDs)在COS-7细胞上有强烈的非特异性吸附,其次是羧基聚乙二醇衍生磷脂修饰的量子点(COOH-PEG-QDs),而甲基聚乙二醇衍生磷脂修饰的量子点(CH3-PEG-QD)在细胞上的非特异性吸附非常小,并且量子点与细胞的作用是一个依赖于量子点的浓度、表面电荷、培育时间与温度,并受培养基中血清影响的耗能过程.
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| 关 键 词： | 量子点 细胞 非特异性吸附 |
Studies of Nonspecific Interaction between Different Functionalized Quantum Dots and Cells |
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| WANG Qing,ZHANG Peng-fei,YANG Xiao-hai,WANG Ke-min and WU Chun-lei.Studies of Nonspecific Interaction between Different Functionalized Quantum Dots and Cells[J].Journal of Hunan University(Naturnal Science),2010,37(5):60-63. |
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| Authors: | WANG Qing ZHANG Peng-fei YANG Xiao-hai WANG Ke-min and WU Chun-lei |
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| Institution: | (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan Univ, Changsha, Hunan 410082, China) |
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| Abstract: | Amino-terminated PEG-phospholipid modified QDs (NH2-PEG-QDs), carboxyl-terminated PEG-phospholipid modified QDs (COOH-PEG-QDs) and methyl-terminated PEG-phospholipid modified QDs (CH3-PEG-QDs) were prepared by encapsulation of QDs into functional PEG-phospholipid micelles, and their nonspecifical binding to COS-7 cells were investigated. The results indicated that the number of QDs adsorbed on the COS-7 cells increased in the order of CH3-PEG-QDs, COOH-PEG-QDs, NH2-PEG-QDs. The interaction between functionalized QDs and cells was affected by particle concentration, surface charge of functionalized QD, incubation time, temperature and the serum in cell culture medium. |
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