忍冬木层孔菌总酚的HPLC分离及其指示性成分分析

对忍冬木层孔菌总酚主要成分进行了分析,为进一步控制总酚质量提供依据.用95%乙醇提取总酚,经大孔树脂吸附,乙醇洗脱制备忍冬木层孔菌总酚,利用高效液相色谱制备主要成分,采用普鲁士蓝紫外-可见分光光度法测定酚含量,用高效液相色谱测定主要成分含量.结果表明总酚中酚的含量达到63.35%,从总酚中分离鉴定原儿茶酸,3,4-二羟基苄叉丙酮两种主要成分,总酚中原儿茶酸的含量为13.1%,3,4-二羟基苄叉丙酮的含量为12.2%.所制备的忍冬木层孔菌总酚符合有效部位质量要求,总酚中原儿茶酸和3,4-二羟基苄叉丙酮均具有抗肿瘤等药理活性,可作为总酚的指示性成分.

The HPLC Separation and Structural Analysis of Index Composition for the Total Phenols in Phellinus Lonicerinus

This paper anlyzes the main marked components and provides the basis for further control the quali-ty of the total phenols in the phellinus lonicerinus.The total phenols were extracted with 95% ethanol,ad-sorbed by macroporous resin and eluted with ethanol.The main components were separated using preparative high performance liquid chromatogram （HPLC）.The content of the total phenols was determined by UV-Vis absorption spectroscopy of Prussian blue dispersion,and the main marked components were tested through HPLC methods.The results show that the content of the total phenols is 63.35% in the phellinus loniceri-nus.The protocatechuic acid and 3,4-dihydroxy benzalacetone were isolated and structural characterized with the corresponding contents of 13.1% and 12.2% respectively.It is concluded that the extracts of the total phenols achieved the quality requirements for the effective component in the phellinus lonicerinus.Both of the protocatechuic acid and 3,4-dihydroxy benzalacetone in the total phenols exhibited good anti-tumor effects, which could be used as the main index composition.