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重金属污染环境来源的玉米POD对Pb2+的胁迫反应
引用本文:张太平,潘伟斌. 重金属污染环境来源的玉米POD对Pb2+的胁迫反应[J]. 华南理工大学学报(自然科学版), 2003, 31(2): 5-8
作者姓名:张太平  潘伟斌
作者单位:华南理工大学,造纸与环境工程学院,广东,广州,510640
基金项目:广州市科技计划资助项目(2001-J011-01);华南理工大学自然科学基金资助项目(E52020)
摘    要:为了探讨植物对重金属污染环境的适应是否产生体内酶系统的耐性机制及其遗传性,以重金属污染环境来源的玉米(Zea mays L.)及其易地栽种子一代(F1)为实验材料,通过酶活性测定与同工酶电泳研究了玉米过氧化物酶对重金属Pb2+的胁迫反应.结果发现,重金属污染环境来源的玉米种群材料在重金属Pb2+的胁迫条件下,与非污染环境来源的玉米材料相比其过氧化物酶具有相对较高的活性,其F1保持相同的趋势;不同玉米种群材料F1过氧化物酶同工酶酶带对重金属Pb2+的胁迫反应在材料间也出现了明显的分化,同时区分出两条对重金属Pb2+胁迫反应的敏感酶带,称之为标志性酶带.

关 键 词:重金属   玉米   过氧化物酶   铅离子
文章编号:1000-565X(2003)02-0005-04
修稿时间:2002-04-18

Reaction of Peroxidase of Maize(Zea mays L.) from Heavy-Metal Polluted Environment to the Stress of Pb2+
Zhang Tai-ping Pan Wei-bin. Reaction of Peroxidase of Maize(Zea mays L.) from Heavy-Metal Polluted Environment to the Stress of Pb2+[J]. Journal of South China University of Technology(Natural Science Edition), 2003, 31(2): 5-8
Authors:Zhang Tai-ping Pan Wei-bin
Abstract:In order to study the tolerance of plants from polluted environment to heavy metals and its heredity, the analysis of enzymatic activity and isozyme electrophoresis were adopted to probe the reaction of peroxidase(POD) of maize(Zea mays L.) samples from heavy-metal polluted environment and their first filial generation (F1) to the stress of Pb2+ . The results showed that POD activity of maize samples from polluted environment was higher than that from clear environment, and their first filial generation (Fl) showed the same trend; POD isozyme differentiated remarkably among maize samples (F1) and two isozymatic bands of POD, which were sensitive to the stress of Pb2+ and were regarded as marked bands.
Keywords:heavy metal  maize  peroxidase  lead ion
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