Neuronal growth inhibitory factor inhibits pheochromocytoma PC12 in vitro |
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Authors: | JI Qingzhou RU Binggen |
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Institution: | (1) National Laboratory of Protein Engineering, College of Life Sciences, Peking University, 100871 Beijing, China |
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Abstract: | Neuronal growth inhibitory factor (GIF), named Metallothioneins-III (MT-III), is the first protein validated to be capable
of inhibiting the growth of neurons in nervous system. We have detected the effects of recombinant GIF on the growth of neuroblastoma
SY5Y and pheochromocytoma PC12 by the MTT (Thiazolyl blue) reduction assay. Recombinant GIF inhibited PC12 in vitro; the inhibitory rate was about 25% when GIF was at 100 mg/L; and the inhibitory rate was about 50% when GIF was at 300 mg/L.
It is shown that PC12 could serve as a proper model for detecting neuronal growth inhibitory activity of GIF. Recombinant
GIF did not inhibit neuroblastoma SY5Y in vitro, a common model of neuroma; it is also shown that GIF could not inhibit neuromata extensively. The reason for GIF inhibiting
PC12 may be that PC12 have some properties of cholinergic neuron. It must play an important role in discovering the mechanism
of GIF’s neuronal growth inhibitory activity. |
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Keywords: | neuronal growth inhibitory factor pheochromocytoma PC12 neuroblastoma SY5Y MTT reduction assay |
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